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Hrp linked goat anti rabbitsecondary

Manufactured by Southern Biotech
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The HRP-linked goat-anti-rabbit secondary is a laboratory reagent used in various immunoassay techniques. It consists of horseradish peroxidase (HRP) conjugated to a goat-derived antibody that specifically binds to rabbit primary antibodies. This product facilitates the detection and visualization of target analytes in samples when used in conjunction with rabbit-based primary antibodies.

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Lab products found in correlation

Odyssey scanner, by LI COR (2 mentions) Chemidoc mp, by Bio-Rad (2 mentions) Cyclin d3 dcs22, by Cell Signaling Technology (2 mentions) Jak3 d7b12, by Cell Signaling Technology (2 mentions) Odyssey block, by LI COR (2 mentions)

2 protocols using hrp linked goat anti rabbitsecondary

1

Western Blotting of Cyclin D3 and JAK3

Check if the same lab product or an alternative is used in the 5 most similar protocols
5–10 × 106 cells were lysed in 50 μL
RIPA buffer with 500 mM NaCl, clarified, and protein normalized by bicinchoninic
acid assay (Pierce). Proteins (20–30 μg) were resolved by
7.5% or 10% SDS-PAGE, transferred to a polyvinylidene fluoride
membrane and blocked with Odyssey Block (LI-COR Biosciences). Blots were probed
for Cyclin D3 (DCS22, 1:1000) or JAK3 (D7B12, 1:1000) (Cell Signaling
Technologies), normalized to tubulin (DM1A, 1:5000, Sigma-Aldrich) and detected
with fluorescent secondary antibodies on an Odyssey Scanner (LI-COR
Biosciences). Alternatively, blots were probed with HRP-linked goat-anti-rabbit
secondary (Southern Biotech, Birmingham AL), developed with Western Lightening
Plus ECL (Pierce) and detected on a ChemiDocMP (Biorad).
Smith G.A., Uchida K., Weiss A, & Taunton J. (2016). Essential biphasic role for JAK3 catalytic activity in IL-2 receptor signaling. Nature chemical biology, 12(5), 373-379.
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2

Western Blotting of Cyclin D3 and JAK3

Check if the same lab product or an alternative is used in the 5 most similar protocols
5–10 × 106 cells were lysed in 50 μL
RIPA buffer with 500 mM NaCl, clarified, and protein normalized by bicinchoninic
acid assay (Pierce). Proteins (20–30 μg) were resolved by
7.5% or 10% SDS-PAGE, transferred to a polyvinylidene fluoride
membrane and blocked with Odyssey Block (LI-COR Biosciences). Blots were probed
for Cyclin D3 (DCS22, 1:1000) or JAK3 (D7B12, 1:1000) (Cell Signaling
Technologies), normalized to tubulin (DM1A, 1:5000, Sigma-Aldrich) and detected
with fluorescent secondary antibodies on an Odyssey Scanner (LI-COR
Biosciences). Alternatively, blots were probed with HRP-linked goat-anti-rabbit
secondary (Southern Biotech, Birmingham AL), developed with Western Lightening
Plus ECL (Pierce) and detected on a ChemiDocMP (Biorad).
Smith G.A., Uchida K., Weiss A, & Taunton J. (2016). Essential biphasic role for JAK3 catalytic activity in IL-2 receptor signaling. Nature chemical biology, 12(5), 373-379.
+ Open protocol
+ Expand

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