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Mouse vegf r1 flt 1 quantikine elisa kit

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The Mouse VEGF R1/Flt-1 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure mouse VEGF R1/Flt-1 levels in cell culture supernates, cell lysates, and tissue homogenates.

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Quantikine ELISA kit (1163 citations) Quantikine ELISA (398 citations) ELISAs (144 citations) Mouse ELISA kits (102 citations) Quantikine kits (80 citations) VEGF ELISA kit (73 citations) Mouse VEGF Quantikine ELISA (5 citations) Quantikines (4 citations) VEGF R1/Flt-1 (3 citations) Mouse Flt-1 (2 citations)

5 protocols using mouse vegf r1 flt 1 quantikine elisa kit

1

Quantification of Soluble VEGF Receptor

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Supernatant from MRC5 expressing s-Flt and control cells was collected. For measurement of s-FLT1 levels in the circulation, blood was collected from the retro-orbital sinus, under anesthesia. Plasma was separated by centrifugation and s-FLT1 levels were measured. s-FLT1 levels were measured using Mouse VEGF R1/Flt-1 Quantikine ELISA Kit (R&D) according to manufacturer’s protocol.
Oren R., Addadi Y., Narunsky Haziza L., Dafni H., Rotkopf R., Meir G., Fishman A, & Neeman M. (2016). Fibroblast recruitment as a tool for ovarian cancer detection and targeted therapy. International Journal of Cancer, 139(8), 1788-1798.
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2

VEGFR1/Flt-1 Serum Quantification

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Flt-1 concentration in mouse serum (diluted 1:5) was quantified by ELISA as per the manufacturer’s instructions (Mouse VEGFR1/Flt-1 Quantikine ELISA kit #MVR100 R&D systems).
Menkhorst E., Santos L.L., Zhou W., Yang G., Winship A.L., Rainczuk K.E., Nguyen P., Zhang J.G., Moore P., Williams M., Lê Cao K.A., Mansell A, & Dimitriadis E. (2023). IL11 activates the placental inflammasome to drive preeclampsia. Frontiers in Immunology, 14, 1175926.
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3

Quantitative Analysis of s-FLT1 in Plasma

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Supernatant from MRC5 expressing s‐Flt and control cells was collected. For measurement of s‐FLT1 levels in the circulation, blood was collected from the retro‐orbital sinus, under anesthesia. Plasma was separated by centrifugation and s‐FLT1 levels were measured. s‐FLT1 levels were measured using Mouse VEGF R1/Flt‐1 Quantikine ELISA Kit (R&D) according to manufacturer's protocol.
Oren R., Addadi Y., Narunsky Haziza L., Dafni H., Rotkopf R., Meir G., Fishman A, & Neeman M. (2016). Fibroblast recruitment as a tool for ovarian cancer detection and targeted therapy. International Journal of Cancer, 139(8), 1788-1798.
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4

VEGFA and FLT1 Quantification in Cell Culture

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Cell culture supernatants were collected at different time points. After centrifugation (1000 rcf, 10 min, 4°C) supernatants were stored at -20°C until further analysis.
Protein levels of VEGFA and FLT1 were determined using Mouse VEGF DuoSet ELISA Kit (R&D Systems; DY493) and Mouse VEGFR1/FLT-1 Quantikine ELISA Kit (R&D Systems; MVR100), in accordance with the instructions provided by the manufacturer. The plates were read using the BIO-TEK Synergy HT Multi-Detection Microplate Reader.
Domokos A., Varga Z., Jambrovics K., Caballero-Sánchez N., Szabo E., Nagy G., Scholtz B., Halasz L., Varadi E., Bene K.P., Mazlo A., Bacsi A., Jeney V., Szebeni G.J., Nagy L, & Czimmerer Z. (2023). The transcriptional control of the VEGFA-VEGFR1 (FLT1) axis in alternatively polarized murine and human macrophages. Frontiers in Immunology, 14, 1168635.
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5

Quantifying Inflammatory Markers in Mice

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Mice plasma concentrations of IL-6, TNF-α, and sFLT-1 were measured using the Mouse TNF-α Quantikine ELISA Kit, the Mouse IL-6 Quantikine ELISA Kit, and the Mouse VEGF R1/FLT-1 Quantikine ELISA Kit (all from R&D Systems, Australia), according to the manufacturer’s instructions. The concentrations were determined according to the absorbances of the samples and standards at a wavelength of 450nm obtained using a microplate reader (BioTek, USA).
Li G., Wei W., Suo L., Zhang C., Yu H., Liu H., Guo Q., Zhen X, & Yu Y. (2021). Low-Dose Aspirin Prevents Kidney Damage in LPS-Induced Preeclampsia by Inhibiting the WNT5A and NF-κB Signaling Pathways. Frontiers in Endocrinology, 12, 639592.
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