Ex vivo wound samples (n = 5) were processed for hematoxylin and eosin (H&E) staining, and analysed using VisiCam (VWR International, UK) software. The epithelial tongue area (µm2) and length (µm) were measured and compared at day 5 post-wounding. To assess cell proliferation, the number of Ki-67+ and DAPI+ cells was counted along the basal layer of the wound epithelial tongue using ImageJ software (National Institutes of Health, Bethesda) and converted to a percentage, as previously described55 (link). Statistical analysis was performed using unpaired Student’s t-test.
Visicam
Manufactured by Avantor
Sourced in United States
VisiCam is a digital imaging system designed for laboratory applications. It captures high-quality images and video of samples or processes within a controlled environment. The core function of VisiCam is to provide visual documentation and analysis capabilities for research and testing activities.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using visicam
1
Wound Epithelial Tongue Measurement
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Ex vivo wound samples (n = 5) were processed for hematoxylin and eosin (H&E) staining, and analysed using VisiCam (VWR International, UK) software. The epithelial tongue area (µm2) and length (µm) were measured and compared at day 5 post-wounding. To assess cell proliferation, the number of Ki-67+ and DAPI+ cells was counted along the basal layer of the wound epithelial tongue using ImageJ software (National Institutes of Health, Bethesda) and converted to a percentage, as previously described55 (link). Statistical analysis was performed using unpaired Student’s t-test.
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Ex vivo wound samples (n = 5) were processed for hematoxylin and eosin (H&E) staining, and analysed using VisiCam (VWR International, UK) software. The epithelial tongue area (µm2) and length (µm) were measured and compared at day 5 post-wounding. To assess cell proliferation, the number of Ki-67+ and DAPI+ cells was counted along the basal layer of the wound epithelial tongue using ImageJ software (National Institutes of Health, Bethesda) and converted to a percentage, as previously described55 (link). Statistical analysis was performed using unpaired Student’s t-test.
2
Microscopic Morphological Analysis
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Morphological features were studied under an Olympus CK40 inverted microscope at 40 x magnification. Images were captured using VisiCam® software (VWR, USA).
3
Cell Morphology Analysis in Culture
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Morphological features of cells in culture were studied under an Olympus CK40 inverted microscope at 20X magnification and images were captured using VisiCam® software (VWR, USA). Cultures were classified as having cobblestone, spindle or mesenchymal morphology.
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