Fitc 3540b

Manufactured by IDEX Corporation

The FITC-3540B is a laboratory instrument manufactured by IDEX Corporation. It is designed to perform fluorescence-based measurements and analyses. The core function of the FITC-3540B is to detect and quantify fluorescent signals from samples.

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Lab products found in correlation

Txred 4040b, by IDEX Corporation (2 mentions) Uplanfln, by Olympus (1 mentions) Ix81 epifluorescence microscope, by Olympus (1 mentions) Dp72 ccd camera, by Olympus (1 mentions) Uplan fln 40 0.75 ph2, by Olympus (1 mentions) Cellsens digital imaging software version 1, by Olympus (1 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions) Orca flash 4.0 v2 camera, by Hamamatsu Photonics (1 mentions) Uplfln 4, by Olympus (1 mentions) Ix81 inverted microscope, by Olympus (1 mentions)

Spelling variants of this product

FITC-3540B-NTE (2 citations)

3 protocols using fitc 3540b

High-Throughput Fluorescence Microscopy Imaging

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After staining, each 96-well plate was imaged by using an Olympus IX81 epifluorescence microscope (×4 objective, N.A. of 0.13, Olympus UPlanFL N; Tokyo, Japan). The imaging area consisted of a grid of either 4 × 3 or 6 × 4 overlapping images covering 47%–54% of the total well area. Well-focused images covering the entire image area were obtained by autofocusing on each image position by using a custom script written in MatLab (Natick, MA). Each sample was imaged by using blue, red, far red, and green filter sets (Chroma ET-DAPI 49000, Semrock TxRed-4040B, Chroma ET-Cy5 49006, and Semrock FITC-3540B, corresponding to the Hoechst 33342, ALP, EDU, and Muc2 stains, respectively) and an exposure time of 150 milliseconds for all channels. The total image acquisition time for a 96-well plate using a 6 × 4 image grid for each well was 456 ± 7.5 minutes.

Wang Y., DiSalvo M., Gunasekara D.B., Dutton J., Proctor A., Lebhar M.S., Williamson I.A., Speer J., Howard R.L., Smiddy N.M., Bultman S.J., Sims C.E., Magness S.T, & Allbritton N.L. (2017). Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells. Cellular and Molecular Gastroenterology and Hepatology, 4(1), 165-182.e7.

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Visualizing Oral Microbial Flora After Brushing

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To visualize the planktonic oral microbial flora 1h after toothbrushing, 1ml of fresh human saliva was incubated with LIVE/DEAD^® BacLight^™ Bacterial Viability Kit containing SYTO^®9 and propidium iodide in a final concentration of 1.67μM (Invitrogen^™) and 1μg/ml Wheat Germ Agglutinin Alexa Fluor^® 350 conjugated (Invitrogen^™) for 10min at 37°C. Mucous fraction obtained by 10min centrifugation at 4500×g was examined using UPlan FLN 40×/0.75 Ph2 and UPlan FLN 100×/1.30 Oil Ph3 on BX51 equipped with BX-URA2 reflected fluorescence system and X-Cite^® 120W metal halide lamp (Olympus). Separate images were taken with DAPI-1160A, FITC-3540B, and TRITC-A BrightLine^® fluorescence filters (Semrock), as well as U-PCD2 phase contrast condenser using DP72 CCD camera and merged with cellSens^® digital imaging software Version 1.3 (Olympus).

Itzek A., Chen Z., Merritt J, & Kreth J. (2016). Effect of salivary agglutination on oral streptococcal clearance by human polymorphonuclear neutrophil granulocytes. Molecular oral microbiology, 32(3), 197-210.

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Time-lapse Imaging of Cells on Microarrays

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Imaging was performed using a motorized Olympus IX81 inverted microscope (Olympus Corporation, Tokyo, Japan) controlled by custom‐built MATLAB software with hardware control using an open‐source Micromanager.²⁴, ²⁶ The microscope was equipped with an MS‐2000 motorized stage (ASI, Eugene, OR) and an Orca‐Flash 4.0 V2 camera (Hamamatsu, Shizuoka, Japan). The objective used was an Olympus UPLFLN 4× (NA 0.13). Fluorescence imaging of Hoechst 33342, mCherry, and GFP, was performed using DAPI (Chroma ET‐DAPI 49000), TxRed (Semrock TxRed‐4040B), and FITC (Semrock FITC‐3540B) filter sets. Time‐lapse images were performed every 2 h for 24 h. The microarray was maintained in focus using a customized software autofocus (MATLAB) employing a modified Laplacian focus algorithm.²¹, ²⁴ During time‐lapse imaging, a custom‐made incubator maintained the cells on the arrays at 37°C, 60% humidity, and 5% CO₂.

Cortés‐Llanos B., Jain V., Cooper‐Volkheimer A., Browne E.P., Murdoch D.M, & Allbritton N.L. (2023). Automated microarray platform for single‐cell sorting and collection of lymphocytes following HIV reactivation. Bioengineering & Translational Medicine, 8(5), e10551.

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