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3 protocols using anti h3k122ac

1

Profiling Histone Modifications and Neuronal Markers

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Antibodies included Anti-H3 (Abcam ab1791), Anti-H3K9ac (Abcam ab4441), Anti-H3K27ac (Abcam ab4729), Anti-H3K122ac (Abcam ab33308), Anti-H4 (Millipore 05-858), Anti-H4K5ac (Millipore 39-584), Anti-H4K12ac (Abcam ab1761), Anti-ACSS2 (T) (Thermo MA5-145810), Anti-ACSS2 (CS) (Cell Signaling 3658), Anti-ACL (Proteintech 15421-1-AP), Anti-α-Tubulin (Sigma T8328), Anti-GAPDH (Fitzgerald Industries 10R-G109A), Anti-KAT3A/CBP (Abcam ab2832), Anti-SNAP25 (Abcam ab5666), Anti-Synaptophysin (Millipore MAB368), Anti-MAP2 C/D (Cell Signaling #8707), Anti-NR4A2 (Santa Cruz sc-991), Anti-NeuN (Millipore ABN78)
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2

Investigating Transcriptional Regulation Mechanisms

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ChIP: anti-H3K27ac (Abcam, ab4729), anti-H3K4me3 (Active Motif, #39159), anti-H3K4me1 (Abcam, ab8895), anti-H3K122Ac (Abcam ab33309), anti-MycN262 (Santa Cruz, sc-764), anti-RNAPol2 N20-X (Santa Cruz, sc-899), anti-YAP1 63.7 (Santa Cruz, sc-101199), anti-TEAD4 (Aviva Systems Biology, ARP38276), anti-BRD4 (Bethyl, A301-985A100), anti-HNF4a (Abcam, ab41898). Normal rabbit/mouse IgG (Santa Cruz, sc-2027) was used as background control. Please note that the anti-TEAD4 (Aviva Systems Biology, ARP38276) was reported to recognize also TEAD1 and TEAD3 (33 (link)). Western-Blot: anti-YAP1 63.7 (Santa Cruz, sc 101199) and anti-TEAD4 (Santa Cruz, sc-101184); anti-HNF4a (Abcam, ab199431), anti-total H3 (Abcam, ab1791); anti-vinculin clone H (SigmaAldrich, V9131); anti-TEAD1 (Cell Signalling, #8526). Goat anti-rabbit HRP (Biorad, #1706515) and Goat anti-mouse HRP (Biorad, #1706516) were used as secondary antibodies. Immunohistochemistry: anti-YAP1 (Cell Signalling, #4911), anti-Ki67 (Thermo Scientific, #9106), anti-Sox9 (Millipore, #5535), anti-HNF4a (Santa Cruz, sc-8987 and Abcam, ab41898).
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3

Profiling Histone Modifications and Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies included Anti-H3 (Abcam ab1791), Anti-H3K9ac (Abcam ab4441), Anti-H3K27ac (Abcam ab4729), Anti-H3K122ac (Abcam ab33308), Anti-H4 (Millipore 05-858), Anti-H4K5ac (Millipore 39-584), Anti-H4K12ac (Abcam ab1761), Anti-ACSS2 (T) (Thermo MA5-145810), Anti-ACSS2 (CS) (Cell Signaling 3658), Anti-ACL (Proteintech 15421-1-AP), Anti-α-Tubulin (Sigma T8328), Anti-GAPDH (Fitzgerald Industries 10R-G109A), Anti-KAT3A/CBP (Abcam ab2832), Anti-SNAP25 (Abcam ab5666), Anti-Synaptophysin (Millipore MAB368), Anti-MAP2 C/D (Cell Signaling #8707), Anti-NR4A2 (Santa Cruz sc-991), Anti-NeuN (Millipore ABN78)
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