Labeled primers

Manufactured by Integrated DNA Technologies

Labeled primers are synthetic oligonucleotides used in various molecular biology techniques, such as PCR and DNA sequencing. They are designed with a fluorescent dye or other label attached, which allows for the detection and tracking of the amplified DNA or sequencing products.

Automatically generated - may contain errors

Lab products found in correlation

Magnesium sulfate, by New England Biolabs (1 mentions) Rox reference dye, by Thermo Fisher Scientific (1 mentions) Thermoscript reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Dntps, by Thermo Fisher Scientific (1 mentions) Evagreen, by Biotium (1 mentions) Betaine, by Merck Group (1 mentions)

Close spelling variants of this product

IRDye 700 labeled primers HPLC-purified fluorescent labeled primers 5′ 32P-end-labeled 18-mer DNA primer Primer and fluorescently labeled internal probe sequences

2 protocols using labeled primers

Reconstitution of Fluorescent Mononucleosomes

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Mononucleosomes were reconstituted from purified, recombinant histones and PCR products containing the 601 positioning sequence as previously described (Lowary and Widom, 1998 (link); Shahian and Narlikar, 2012 (link)). Fluorescent DNA labels were introduced by PCR with labeled primers (Integrated DNA Technologies, IBA Life Sciences) and histone labels were introduced prior to octamer refolding using dye maleimides, similar to previously described (Shahian and Narlikar, 2012 (link)).

Leonard J.D, & Narlikar G.J. (2015). A Nucleotide-Driven Switch Regulates Flanking DNA Length Sensing by a Dimeric Chromatin Remodeler. Molecular cell, 57(5), 850-859.

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Quantitative RT-PCR Protocol

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Bst and Taq polymerases, associated reaction buffers, and magnesium sulfate were purchased from New England Biolabs (Ipswich, MA). Betaine was purchased from Sigma Aldrich (St. Louis, MO), dNTPs and Thermoscript Reverse Transcriptase from Life Technologies (Carlsbad, CA). Superscript III Platinum One-Step quantitative kit, EvaGreen from Biotium (Hayward, CA) and ROX reference dye were purchased from Invitrogen (Carlsbad, CA). All primers, labeled primers, and probes were purchased from Integrated DNA Technologies (Coralville, IA).

Linnes J.C., Rodriguez N.M., Liu L, & Klapperich C.M. (2016). Polyethersulfone improves isothermal nucleic acid amplification compared to current paper-based diagnostics. Biomedical microdevices, 18(2), 30.

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