β actin sc 1616 hrp

Manufactured by Santa Cruz Biotechnology

β-actin (sc-1616-HRP) is a horseradish peroxidase (HRP) conjugated antibody that specifically recognizes the β-actin protein. β-actin is a ubiquitous cytoskeletal protein involved in various cellular processes. This antibody can be used for detection of β-actin in Western blotting applications.

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Lab products found in correlation

Pcdna p63, by Addgene (2 mentions) Pccl nr4a2, by Addgene (2 mentions) Trichostatin a tsa, by Merck Group (1 mentions) Suberoylanilide hydroxamic acid saha, by Merck Group (1 mentions) Dibenzazepine dbz, by Merck Group (1 mentions) Notch1, by Santa Cruz Biotechnology (1 mentions) Valproic acid sodium salt vpa, by Merck Group (1 mentions)

Spelling variants of this product

β-actin (5071 citations) Sc-1616 (129 citations) Actin (sc-1616) (38 citations) β-actin (sc-1616) (24 citations) β-actin-HRP (23 citations) Actin-HRP (17 citations) Sc-1616-HRP (11 citations) Actin-HRP (sc-1616; (2 citations)

4 protocols using β actin sc 1616 hrp

Notch Signaling and Histone Acetylation Regulation

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Valproic acid sodium salt (VPA), DAPT (LY-374973, N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), dibenzazepine (DBZ) and trichostatin A (TSA) and suberoylanilidehydroxamic acid (SAHA), were purchased from Sigma (St. Louis, MO). Antibodies of p21 (sc-756), p63 (sc-8343), COX2 (sc-7951), histone 4 (H4, sc-10810), HDAC4 (sc-11418), acetylation of histone 4 (AcH4, sc-8660-R), Notch1 (sc-23299), NR4A2 (Nurr1, N-20, sc-991), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The plasmids expressing the intracellular domains of the four Notch receptors (ICN1, ICN2, ICN3, and ICN4) were gifts from Dr. Wu (University of Florida). The plasmids with the genes NR4A2 (Nurr1) and p63 were obtained from Addgene (www.addgene.org), including pcDNA-p63 (27008), pCCL-NR4A2 (plasmid 35000) and the control pCCL (10881).

Zhu B., Sun L., Luo W., Li M., Coy D.H., Yu L, & Yu W. (2017). Activated Notch signaling augments cell growth in hepatocellular carcinoma via up-regulating the nuclear receptor NR4A2. Oncotarget, 8(14), 23289-23302.

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Notch Signaling Pathway Modulation

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The plasmids expressing the intracellular domains of the four Notch receptors (ICN, ICN2, ICN3, and ICN4) and dnMAML (DNL) were gifts from Dr. Wu (University of Florida). The plasmids with the genes NR4A2 (Nurr1), Vinculin (VCL), THBS1 (TSP1), p63 and Twist were obtained from Addgene (www.addgene.org), including pCCL-NR4A2 (plasmid 35000) and the controls pCCL (10881), pmEN1-VCL (54304), pmEN1 (53976), pBabe-Twist1 (1783), pBabe (10668), pcDNA-THBS1 (12405) and pcDNA-p63 (27008). Antibodies of p21 (Cat. No.: sc-756), p63 (sc-8343), c-Myc (sc-788), NR4A2 (sc-990), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Sun L., Liu M., Sun G.C., Yang X., Qian Q., Feng S., Mackey L.V, & Coy D.H. (2016). Notch Signaling Activation in Cervical Cancer Cells Induces Cell Growth Arrest with the Involvement of the Nuclear Receptor NR4A2. Journal of Cancer, 7(11), 1388-1395.

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Antibody Detection Protocol

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See supplementary material and methods section. The following antibodies and conditions were used: Gnmt (AP1076b; 1:500; Abgent, San Diego, CA), β-Actin (sc-1616 HRP; 1:3000; Santa Cruz Biotechnology, Dallas, TX).

Wagner M., Choi S., Panzitt K., Mamrosh J.L., Lee J.M., Zaufel A., Xiao R., Wooton-Kee R., Ståhlman M., Newgard C.B., Borén J, & Moore D.D. (2015). LRH-1 is a critical determinant of methyl-pool metabolism. Hepatology (Baltimore, Md.), 63(1), 95-106.

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Valproic Acid Regulates Epigenetics

Cited 1 time

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Valproic acid sodium salt (VPA) was purchased from Sigma (St. Louis, MO). Antibodies to SSTR2 (Cat. No.: sc-11609), p21 (sc-756), p63 (sc-8343), histone 4 (H4, sc-10810), HDAC4 (sc-11418), acetylation of histone 4 (AcH4, sc-8660-R), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), with Notch1 (Cat. No.: 4380) from Cell Signaling Technology (Danvers, MA). The plasmids are the kind gifts from Dr. Wu (University of Florida). The cytotoxic CPT-SST was synthesized in our laboratories 38 (link).

Sun G., Mackey L.V., Coy D.H., Yu C.Y, & Sun L. (2015). The Histone Deacetylase Inhibitor Vaproic Acid Induces Cell Growth Arrest in Hepatocellular Carcinoma Cells via Suppressing Notch Signaling. Journal of Cancer, 6(10), 996-1004.

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