Rabbit anti tenomodulin

Manufactured by Abcam

Sourced in United Kingdom

Rabbit anti-tenomodulin is a primary antibody that binds to the tenomodulin protein. Tenomodulin is a type II transmembrane glycoprotein that is involved in the regulation of tenocyte differentiation and tendon formation. This antibody can be used in various research applications to detect and study the tenomodulin protein.

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Lab products found in correlation

Alexa fluor 488 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Mouse anti cd44, by BD (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Fv3000, by Olympus (1 mentions) Mouse anti collagen 1 antibody, by Abcam (1 mentions) Mouse anti aggrecan, by Novus Biologicals (1 mentions) Rabbit anti collagen 2, by Abcam (1 mentions)

Close spelling variants of this product

Rabbit anti- (5 citations) Anti-tenomodulin (3 citations)

2 protocols using rabbit anti tenomodulin

Immunofluorescence Staining of Tenomodulin, CD44, and CD90

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TDCs were seeded at a cell density of 5 × 10⁴ cells/well and incubated at 37 °C at 5% CO₂. After 24 h incubation, cells were fixed at 37 °C for 10 min with 4% formaldehyde (pH 7.4). They were then washed twice with PBS and permeabilized at room temperature for 10 min. Next, cells were blocked in blocking solution (2% bovine serum albumin in PBS) for 1 h at room temperature, incubated overnight with primary antibodies diluted in blocking solution at 4 °C, washed three times for 5 min with PBS, incubated with secondary antibody at RT for 2 h, and washed four times for 5 min with PBS. The primary antibodies used for immunostaining and their dilutions were as follows: rabbit anti-tenomodulin (1:50, Abcam, Cambridge, UK), mouse anti-CD44 (1:50, BD Biosciences), mouse anti-90(1:50, BD Biosciences). The secondary antibodies used were Alexa Fluor 488-conjugated goat anti-mouse IgG (1:100, Thermo, Waltham, US) and Alexa Fluor 555-conjugated goat anti-rabbit IgG (1:100, Thermo).

Hsiao M.Y., Lin P.C., Liao W.H., Chen W.S., Hsu C.H., He C.K., Wu Y.W., Gefen A., Iafisco M., Liu L, & Lin F.H. (2019). The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor. International Journal of Molecular Sciences, 20(14), 3437.

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Immunofluorescent Staining of Engineered Constructs

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Constructs were fixed in 4% paraformaldehyde (Sigma), 1% sucrose (Sigma) in PBS. After washing with PBS, constructs were blocked using 5% v/v goat serum and 1% w/v BSA in PBS for 1 h, then incubated with mouse-anti-collagen I antibody (1:500, Abcam), mouse-anti-aggrecan (1:500, Novus Biologicals, Centennial, CO), or mouse-anti-osteopontin (1:250, Abcam). Secondary staining was done immediately after each primary antibody using goat-anti-mouse antibody conjugated with Alexa Fluor 488 (1:500, Invitrogen). A second set of primary antibodies was incubated with rabbit-anti-collagen II (1:200, Abcam), rabbit-anti-decorin (1:100, Abcam), or rabbit-anti-tenomodulin (1:200, Abcam) overnight. They were then secondary stained with goat-anti-rabbit antibody conjugated with Alexa Fluor 594 (Invitrogen) following the manufacturer’s instructions. Following the final secondary stain, nuclei were counter-stained with DAPI. PBS washes were done between each antibody solution change. Fluorescent images were taken on a Nikon Eclipse Ti2 Series microscope and confocal images were taken on an Olympus FV3000. Multi-phasic constructs had images taken in three distinct regions. Image processing and analysis were done using FIJI software [30 (link)].

Hurley-Novatny A., Arumugasaamy N., Kimicata M., Baker H., Mikos A.G, & Fisher J.P. (2020). Concurrent multi-lineage differentiation of mesenchymal stem cells through spatial presentation of growth factors. Biomedical materials (Bristol, England), 15(5), 055035.

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