Eagle s basal medium

C3H10T1/2 cells were purchased from the Health Science Research Resources Bank. HEK293, HEK293T, AGS, and H146 cells were purchased from the American Type Culture Collection. C3H10T1/2 cells were maintained in Eagle’s basal medium (Sigma-Aldrich) supplemented with 10% foetal bovine serum (FBS). HA-FLAG-GLI1-expressing HEK293 cells were maintained in 293SFM medium (ThermoFisher Scientific) or Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS. HEK293 and HEK293T cells were maintained in DMEM supplemented with 10% FBS. AGS cells were maintained in DMEM/F-12 medium supplemented with 10% FBS. H146 cells were maintained in RPMI 1640 medium supplemented with 10% FBS.

TAX (purity ≥ 98%), 5-azadeoxycytidine (5-aza, purity ≥ 97%), trichostatin A (TSA, purity ≥ 98%), bacteriological agar, Eagle’s basal medium (BME) and TPA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s modification of eagle’s medium (DMEM), minimum essential medium (MEM) and trypsin-EDTA solution were obtained from Gibco Laboratories (Gaithersburg, MD, USA). The primary antibodies anti-Nrf2, anti-HO-1, anti-NQO-1 and anti-β-actin were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The primary antibodies anti-DNMT (DNMT1, DNMT3a and DNMT3b) were obtained from IMGENEX (San Diego, CA, USA). The primary antibodies anti-HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7 and HDAC8) were obtained from Cell Signaling Technology (Danvers, MA, USA).