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Sml2234

Manufactured by Merck Group
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Sourced in China

The SML2234 is a laboratory equipment product manufactured by Merck Group. It serves as a centrifugation device used to separate different components of a liquid sample based on their densities.

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Lab products found in correlation

Erastin e7781, by Merck Group (1 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (1 mentions) Arachidonic acid, by Cayman Chemical (1 mentions) Linoleic acid, by Cayman Chemical (1 mentions) Oleic acid, by Cayman Chemical (1 mentions) Eicosapentaenoic acid, by Cayman Chemical (1 mentions) Erastin, by Merck Group (1 mentions) Docosahexaenoic acid dha, by Cayman Chemical (1 mentions) Docosapentaenoic acid dpa, by Cayman Chemical (1 mentions) Fatty acid free bsa, by Merck Group (1 mentions) Ml210, by Merck Group (1 mentions) Glomax multi plus, by Promega (1 mentions) Cck 8 solution, by Beyotime (1 mentions) Tms 001 c, by Merck Group (1 mentions)

4 protocols using sml2234

1

LDH Cytotoxicity Assay for Ferroptosis

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LDH is a marker found in the cytoplasm. When the cell membrane is broken, this molecule is released from the cytoplasm of the cell into the culture medium. The amount of LDH leaked was determined using an LDH cytotoxicity detection kit (CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega)). Approximately 6000 HEI-OC1 and Nrf2 KO cells/well were cultivated separately in 96-well plates overnight and then treated with or without cisplatin. According to the manufacturer's instructions, 100 μL of fresh reaction solution was added to each well for 30 min of incubation at room temperature (RT) after the different treatments, and a microplate reader (BioTek, Epoch) was used to estimate LDH release by measuring the absorbance at 492 nm. The classical ferroptosis inducers 1S,3R-RSL3 (#SML2234) and erastin (#E7781) were purchased from Sigma.
Wang W., Ma P., Gao W., Lu P., Ding X., Chen J., Yuan H, & Lu L. (2022). Nrf2 Knockout Affected the Ferroptosis Signaling Pathway against Cisplatin-Induced Hair Cell-Like HEI-OC1 Cell Death. Oxidative Medicine and Cellular Longevity, 2022, 2210733.
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2

Ferroptosis Induction and Inhibition Assays

Cited 2 times
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The following compounds were obtained from Sigma-Aldrich: ML210 (SML0521, purity ≥ 98%, used at wide concentration range), 1S, 3R-RSL3 (RSL3, SML2234, purity ≥ 98%, used at wide concentration range), ferrostatin-1 (Fer-1, SML0583, purity ≥ 95%, used at 5 μM), erastin (E7781, purity ≥ 98%, used at wide concentration range) and L-buthionine-[S,R]-sulfoximine (BSO, B2515, purity ≥ 97%, used at wide concentration range). Free fatty acids, including oleic acid (OA, C18:1), linoleic acid (LA, C18:2), a-linolenic acid (ALA, C18:3), arachidonic acid (AA, C20:4), eicosapentaenoic acid (EPA, C20:5), docosapentaenoic acid (DPA, C22:5), and docosahexaenoic acid (DHA, C22:6) were purchased from Cayman Chemicals and conjugated with fatty-acid free BSA (Sigma-Aldrich) using previously described protocols51 , and treated cells at 20 μM for 3 days in the viability assays. The following compounds were prepared according to known literature procedures: FINO236 (link) and FIN5634 (link). Alkyne analogs of ML210 and RSL3 (ML210-yne and RSL3-yne, respectively) were synthesized as previously described15 (link).
Zou Y., Li H., Graham E.T., Deik A.A., Eaton J.K., Wang W., Sandoval-Gomez G., Clish C.B., Doench J.G, & Schreiber S.L. (2020). Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis. Nature chemical biology, 16(3), 302-309.
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3

Chondrocyte Viability Assay with RSL3 and Pioglitazone

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Chondrocytes were seeded into 96-well plates (4000/well) and incubated overnight in 100 µL/well of culture medium. For the experiment, the media was replaced with 100 µL of media containing different drugs. After RSL3 (SML2234, Sigma) or/and pioglitazone (PHR1632, Sigma) treatment, the medium was changed to a new medium (100 µL) containing 10 µL CCK-8 solution (C0038, Beyotime, Shanghai, China). Following 1 h of incubation, a microplate reader (GloMax Multi Plus, Promega, Sunnyvale, CA, USA) was used to measure the optical density (450 nm).
Xue X., Dai T., Chen J., Xu Y., Yang Z., Huang J., Xu W., Li S, & Meng Q. (2023). PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis. Journal of Orthopaedic Surgery and Research, 18, 620.
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4

Ferroptosis Induction in MCA205 Murine Fibrosarcoma

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Murine fibrosarcoma MCA205 cells, which are used in this study, are often used for the analysis of the immunogenicity of dead cancer cells and the development of targeted cancer immunotherapies [2] . MCA205 cells were cultured in RPMI 1640 with L-glutamine (LONZA, BE12-702F) supplemented with 10% FBS (FisherScientific, 11591821), 1% Penicillin/streptomycin (LONZA, DE17-602E), 1% Non-Essential Amino Acids (Sigma-Aldrich, TMS-001-C) and 1% Sodium-Pyruvate. Induction of ferroptosis in MCA205 cells is executed by adding 2.5µM Ras-selective lethal 3 (RSL3, Sigma-Aldrich, SML2234).
Van der Meeren L., Efimova I., Demuynck R., Parakhonskiy B., Krysko D.V, & Skirtach A.G. (2023). Mechanobiology of Ferroptotic Cancer Cells as a Novel "Eat-Me" Signal: Regulating Efferocytosis through Layer-by-Layer Coating. Advanced healthcare materials, 12(28).
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