IHC staining was conducted to examine the altered expression of LAIR-1 in tumors and normal tissues. Formalin-fixed and paraffin-embedded sections were deparaffinized and rehydrated. Antigen retrieval was performed in a microwave at 98°C for 30 min. The sections were treated with 3% hydrogen peroxide for 5 min, and then blocked with 1% bovine serum albumin (AR1006; Boster Biological Technology, Ltd., Wuhan, China) for 30 min. The sides were incubated with monoclonal mouse anti-human LAIR-1 (ab14826; Abcam, Cambridge, UK) at 1:500 dilution at 4°C overnight. Mouse IgG (BA1046; Boster Biological Technology, Ltd.) served as the negative control. The biotinylated anti-mouse secondary antibody (BA1001; Boster Biological Technology, Ltd.) was incubated the following day with the sides for 20 min at room temperature, followed by further treatment with a streptavidin-alkaline phosphatase complex (Boster Biological Technology, Ltd.) for 20 min. The signal was detected using the BCIP/NBT substrate solution (Boster Biological Technology, Ltd.).
Ba1046
Manufactured by Boster Bio
Sourced in China
BA1046 is a laboratory equipment product. It is a multi-purpose device designed for use in various scientific and research applications. The core function of BA1046 is to perform a specific task or procedure, but a detailed description of its intended use cannot be provided in an unbiased and factual manner without potential for extrapolation.
Automatically generated - may contain errors
2 protocols using ba1046
1
Immunohistochemical Analysis of LAIR-1 Expression in Tumors
2
Immunohistochemical Analysis of WIP1 in Mouse Ovaries
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The collected mouse ovaries were embedded in paraffin and serially sectioned (5 μm). As previously described [31 (link)], the sections were incubated with primary antibody (anti-WIP1 (F-10) sc-376257, 1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4 °C. The sections were incubated with a secondary antibody (HRP-labeled goat Anti-rabbit/mouse IgG (H + L), Servicebio, GB23204, 1:200) for 60 min at 37 °C on the next day. Then, the sections were visualized with DAB-HRP chromogenic agent (DAKO, K5007). The negative control was section incubated with mouse or rabbit IgG (H + L) (2 μg/mL, BA1046, BA1044, BOSTER, Wuhan, China). Microscopy and the images were obtained by microscope (version 1.8.1, Olympus, Tokyo, Janpan) with the cellSens Dimension software. Image Pro Plus software was used to evaluate the relative expression.
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