This assay was executed with Annexin V FITC and propidium iodide (PI) to measure the apoptosis of the circulating blood cells. The apoptotic analysis was dedicated to discriminating between early and late apoptotic cells, as well as necrotic cells. According to standard protocol (24 (link)) which was modified by (33 (link)), the apoptosis of the treated and the untreated cells was assessed through a flow cytometer instrument (BD Biosciences, USA). The gating of the required cell population was carried out according to the standard procedures of the flow cytometer.
Flow cytometer instrument
Manufactured by BD
Sourced in United States
A flow cytometer is an analytical instrument used to detect and measure physical and chemical characteristics of particles or cells suspended in a fluid as they pass through a laser beam. It is designed to analyze the properties of individual cells or particles within a heterogeneous mixture.
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Lab products found in correlation
4 protocols using flow cytometer instrument
1
Apoptosis Measurement by Flow Cytometry
2
Flow Cytometric Evaluation of CD44 Expression
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The concentration of cancer cells was adjusted to 1 × 106/ml. The cells were washed three times with ice-cold PBS and incubated with FITC-labeled mouse anti-human CD44 antibody (BD Biosciences, 560977) for 30 min in the dark and then analyzed using a flow cytometer instrument (BD Biosciences).
3
Apoptosis Assay with ATO and Sorafenib
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Cells were treated with indicated concentrations of ATO and Sorafenib for 48h, then fixed in 70% in ethanol and dye by PI. Cells were assessed by BD flow cytometer instrument analyzed with flowjo program. The apoptotic cell fraction could predict from hypodiploid sub-G0/G1 DNA fraction.
4
Apoptosis Assay with ATO and Sorafenib
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Cells were treated with indicated concentrations of ATO and Sorafenib for 48h, then fixed in 70% in ethanol and dye by PI. Cells were assessed by BD flow cytometer instrument analyzed with flowjo program. The apoptotic cell fraction could predict from hypodiploid sub-G0/G1 DNA fraction.
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