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Ptfe membrane syringe filter

Manufactured by Avantor
Sourced in United States

The PTFE (polytetrafluoroethylene) membrane syringe filter is a laboratory equipment designed to filter samples prior to analysis. It features a PTFE membrane that effectively removes particulates and impurities from liquids, ensuring the sample's purity and integrity for further testing or processing.

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3 protocols using ptfe membrane syringe filter

1

Quantifying α-Tocopherol in Microgreens

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The α-tocopherol content was evaluated using high-performance liquid chromatography (HPLC) on a Pinnacle II Silica column (Restek, USA; 5-μm particle size; 150 mm × 4.6 mm), as described previously (Fernandez-Orozco et al., 2003 (link)). Tocopherols were extracted from fresh plant tissues using pure hexane (1:10) by centrifugation at 349 × g for 5 min. The supernatant was filtered through a 0.45-μm polytetrafluoroethylene (PTFE) membrane syringe filter (VWR International, USA). The HPLC 10A system, equipped with an RF-10A fluorescence detector (Shimadzu, Japan), was used for analysis. Peaks were detected at an excitation wavelength of 295 nm and an emission wavelength of 330 nm. The mobile phase (0.5% isopropanol in hexane) was used at a flow rate of 1 ml min−1. The α-tocopherol was identified according to the analytical standard. The α-tocopherol content is expressed per gram dry weight of microgreens.
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2

Sediment Pigment Extraction Procedure

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Two sediment samples (1 cm-depth) were collected in each plot of all the areas with a syringe (50 mL), kept in a cooler box during transport, and stored at -20°C until storage at -80°C at the laboratory. Before extraction, sediment samples were freeze-dried and homogenized. The pigments from 0.5 g of dry sediments were extracted with 5 mL of methanol buffered with 2% of 1 M-ammonium acetate (Sigma-Aldrich, France). After 2 min in an ultrasound cold bath and at maximum power, samples were kept in the dark at -20°C for 15 min before centrifugation (High Conic Rotor, Thermo Scientific, 3220 g, 2°C, and 5 min). Supernatants were collected and the pellets were re-extracted as described above. The pooled supernatants were filtered on 0.2 μm PTFE membrane syringe filter (Ø 13 mm, VWR International, United States) and stored a few days at -80°C before High Performance Liquid Chromatography (HPLC) analysis. To prevent degradation of pigments, extractions were performed under dark conditions and samples stored on ice during handling.
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3

HPLC Analysis of Tocopherol Profiles

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Analysis of tocopherols was performed by HPLC according to Brazaityte et al.’s [25 (link)] methodology with some modifications. About 1 mg of oil was weighed in an Eppendorf tube, and then 1 mL of n-hexane with 1% of BHT was added to the tube. Afterward, the samples were filtrated through a 0.45 µm polytetrafluoroethylene (PTFE) membrane syringe filter (VWR International, Radnor, PA, USA) and were analyzed by HPLC/FLD (fluorescence detector) (Agilent Technologies, Santa Clara, CA, USA). The HPLC measurements were performed using a normal phase column (Phenomenex Luna Silica, 5 μm, 250 mm × 4.6 mm). The HPLC 10A system, equipped with an RF-10A fluorescence detector (Shimadzu, Japan), was used for analysis. Peaks were detected at an excitation wavelength of 295 nm and an emission wavelength of 330 nm. The mobile phase (0.5% isopropanol in hexane) was used at a flow rate of 1 mL min−1. The α-tocopherol, γ-tocopherol, and δ-tocopherol were identified according to the analytical standard. The α-tocopherol, γ-tocopherol, and δ-tocopherol content were expressed per 100 g of oil.
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