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U plex biomarker kit

Manufactured by Mesoscale
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The U-PLEX Biomarker kit is a multiplex assay developed by Mesoscale to simultaneously measure multiple biomarkers in a single sample. The kit utilizes Mesoscale's proprietary U-PLEX technology to enable the quantitative detection of target analytes.

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Lab products found in correlation

Ms2400 imager, by MSD (1 mentions) Bio plex pro human cytokine assay, by Bio-Rad (1 mentions) Meso quickplex sq 120, by Mesoscale (1 mentions)

Close spelling variants of this product

U-PLEX kits U-PLEX Biomarker kits

2 protocols using u plex biomarker kit

1

Maternal Cytokine Analysis in Caspr2 Autoimmunity

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Cytokine analysis was performed on 8 maternal Caspr2 and Control immunized serum pulled to 4 samples each, using a custom U-PLEX Biomarker kit (Meso Scale Discovery (MSD), Rockville, MD), including U-PLEX Mouse IFN-γ, U-PLEX Mouse IL-6, U-PLEX Mouse IL-17A, U-PLEX Mouse IL-23 and U-PLEX Mouse TNF-α. MSD plates were analyzed on the MS2400 imager (MSD). The assay was performed according to the manufacturer’s instructions. All standards and samples were measured in duplicate.
Bagnall-Moreau C., Huerta P.T., Comoletti D., La-Bella A., Berlin R., Zhao C., Volpe B.T., Diamond B, & Brimberg L. (2020). In utero exposure to endogenous maternal polyclonal anti-Caspr2 antibody leads to behavioral abnormalities resembling autism spectrum disorder in male mice. Scientific Reports, 10, 14446.
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2

Quantifying Cytokine Levels in Murine and Human Sera

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To measure cytokine titres in murine serum samples, we used a U-PLEX Biomarker kit (Meso Scale Discovery), as per the manufacturer’s instructions. Samples were analysed using a MESO QuickPlex SQ 120 (Meso Scale Discovery). IL-17A titres in human serum samples were quantified using a multiplex cytokine panel (Bio-Plex Pro Human Cytokine Assay, BioRad) and a LuminexCorp Luminex 100 machine as per the manufacturer’s instructions.
Sinton M.C., Chandrasegaran P.R., Capewell P., Cooper A., Girard A., Ogunsola J., Perona-Wright G., M Ngoyi D., Kuispond N., Bucheton B., Camara M., Kajimura S., Bénézech C., Mabbott N.A., MacLeod A, & Quintana J.F. (2023). IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic murine Trypanosoma brucei infection. Nature Communications, 14, 7070.
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