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2 protocols using nci h1355

1

Culturing Non-Small Cell Lung Cancer Cell Lines

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All human non-small cell lung cancer cell lines (NCI-H1437 (ATCC CRL-5872), A549 (ATCCCCL-185), NCI-H460 (ATCC HTB-177), NCI-H1355 (ATCC CRL-5865), NCI-H1650 (ATCCCRL-5883), NCI-H1975 (ATCC CRL-5908), NCI-H358 (ATCC CRL-5807), NCI-H2009 (ATCCCRL-5911)) were either purchased directly from ATCC or were a gift from Dr. Michael Bassik’s laboratory, who previously purchased them from ATCC. Human cell lines were cultured in RPMI media supplemented with 10% FBS, 1% penicillin-streptomycin-glutamate, and 0.1% amphotericin. All human cell lines tested negative for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza).
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2

NSCLC Cell Lines Authentication

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The human NSCLC cell lines, A549, NCI-H23, NCI-H358, NCI-H460, NCI-H647, NCI-H1355, and NCI-H1792 were purchased from ATCC and authenticated with short tandem repeat (STR) profiling by Genesky Biotechnologies Inc (Shanghai, China). A549 cells were maintained in Ham’s F-12 Medium (Corning, NY, USA), and the remaining cell lines were maintained in RPMI 1640 medium (Corning), supplemented with sodium pyruvate (1 mM) and glucose (2500 mg/L). All culture media were supplemented with 10% heat-inactivated fetal bovine serum (FBS).
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