S 2 3 bis palmitoyloxy 2 rs propyl n palmitoyl r cys ser lys4 oh pam3cys

Manufactured by InvivoGen

S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-Ser-Lys4-OH (Pam3Cys) is a synthetic triacylated lipopeptide compound. It acts as a toll-like receptor 2 (TLR2) agonist.

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Lab products found in correlation

Phopho akt ser473 c d9e, by Cell Signaling Technology (2 mentions) Mpges inhibitor cay10526, by Cayman Chemical (2 mentions) Pge2 ep4 antagonist bgc 20 1531 ep2 antagonist pf 04418948 ep3 antagonist l 798 106 akt1 2 inhibitor akti 1 2 pka inhibitor h89 dihydrochloride, by Bio-Techne (2 mentions) Epac inhibitor esi 09, by Bio-Techne (2 mentions) Polyinosinic polycytidylic acid poly 1 c, by InvivoGen (2 mentions) Anti caspase 1 p20, by Adipogen (2 mentions) Antibodies against total p38, by Cell Signaling Technology (2 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Anti tlr2 antibody, by BioLegend (1 mentions) Phospho p65, by Cell Signaling Technology (1 mentions) Total p65, by Cell Signaling Technology (1 mentions) Necrostatin, by Santa Cruz Biotechnology (1 mentions) C12 ie dap, by InvivoGen (1 mentions) L18 mdp, by InvivoGen (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions)

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Pam3Cys (31 citations)

4 protocols using s 2 3 bis palmitoyloxy 2 rs propyl n palmitoyl r cys ser lys4 oh pam3cys

Immunoblotting and Pharmacological Inhibition

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Antibodies against cPLA2 (D49A7), phospho-cPLA2 (D4I2A), IRF3 (D83B9), phospho-IRF3(4D4G), phospho-TBK-1 (D52C2), phospho-JNK (81E11), phospho-ERK1/2 (D13.14.4E), p38 (D13E1), phospho-p38 (D3F9), IκBα (44D4 and 9242), Syk (2712), phospho-Syk TYR525/526 (C87C1), AKT (11E7), and phopho-AKT Ser473 c(D9E) were obtained from Cell Signaling. Anti-Caspase-1 p20 was obtained from Adipogen. Protein-free phenol/water-extracted Escherichia-coli K235 LPS was prepared as described previously ^{65 (link)}. S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-Ser-Lys₄-OH (Pam3Cys) and polyinosinic:polycytidylic acid (poly I:C; VacciGrade) was purchased from InvivoGen. Purified Adenylate Cyclase Toxin (AC) from Bordetella pertussis was a gift of E. Hewlett (Univ. of Virginia) and has been described previously^{39 (link)}. The mPGES inhibitor (CAY10526) was obtained from Cayman Chemical. PGE₂, EP4 antagonist (BGC 20–1531), EP2 antagonist (PF-04418948), EP3 antagonist (L-798,106), AKT1&2 inhibitor (AKTi-1/2), PKA inhibitor (H89 dihydrochloride), and EPAC inhibitor (ESI 09) were obtained from Tocris Bioscience.

Perkins D.J., Richard K., Hansen A.M., Lai W., Nallar S., Koller B, & Vogel S.N. (2018). Autocrine-Paracrine Prostaglandin E2 Signaling Restricts TLR4 internalization and TRIF Signaling. Nature immunology, 19(12), 1309-1318.

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Molecular Signaling Pathway Analysis

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Antibodies against total p38, phospho-p38, total p65, and phospho-p65 were purchased from Cell Signaling (Danvers, MA). Anti-TLR2 antibodies used in Flow Cytometry analysis were purchased from Biolegend (San Diego, CA). S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]- N-palmitoyl-(R)-Cys-Ser-Lys₄-OH (Pam3Cys) was purchased from InVivogen (San Diego, CA). Purified, mammalian-expressed murine and human recombinant IFN-β (rIFN-β) was purchased from PBL (Piscataway, NJ).

Richard K., Vogel S.N, & Perkins D.J. (2016). Type I interferon Licenses Enhanced Innate Recognition and Transcriptional Responses to Franciscella tularensis LVS. Innate immunity, 22(5), 363-372.

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Immunomodulatory Factors and Signaling

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Antibodies against total p38, phospho-p38, total p-38, phospho-ERK1/2, total ERK 1/2, total p65, phospho p65, and total IL-1β were purchased from Cell Signaling (Danvers, MA). Anti-IL-10 and anti-IL-27 neutralizing antibodies were purchased from e-Bioscience (San Diego, CA). All antibodies used in Flow Cytometry were purchased from Biolegend (San Diego, CA). Recombinant murine IL-10 and IL-27 was purchased from Biolegend. Protein-free phenol/water extracted Escherichia-coli K235 LPS was prepared as described elsewhere (23 (link)). S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-Ser-Lys₄-OH (Pam3Cys) was purchased from InVivogen (San Diego, CA). Purified mammalian-expressed recombinant IFN-β was purchased from PBL (Piscataway NJ). Recombinant murine IFN-γ was kindly provided by Genentech, Inc. (San Francisco, CA). Necrostatin was purchased from Santa Cruz (Santa Cruz, CA). L18-MDP and C12-iE-DAP were purchased from Invivogen (San Diego, CA).

Perkins D.J., Rajaiah R., Tennant S.M., Ramachandran G., Higginson E.E., Dyson T.N, & Vogel S.N. (2015). Salmonella Typhimurium Co-opts the Host Type I Interferon System to Restrict Macrophage Innate Immune Transcriptional Responses Selectively. Journal of immunology (Baltimore, Md. : 1950), 195(5), 2461-2471.

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Immunoblotting and Pharmacological Inhibition

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