Cell proliferation and viability were analyzed by the Celigo imaging cytometry system and MTT assay, respectively. The fluorescence intensity of entire wells, of transfected A549 cells was detected, and the number of cells was automatically calculated by the Celigo imaging cytometry system (Nexcelom Bioscience LLC). Cell viability was measured using a Cell Viability kit (MTT; Roche Diagnostics) solubilized in 150 µl dimethyl sulfoxide according to the manufacturer's instructions. The absorption of the solution was measured at 570 nm at various time points.
Cell viability kit mtt
Manufactured by Roche
Sourced in Switzerland
The Cell Viability kit (MTT) is a colorimetric assay used to measure cell metabolic activity. It utilizes the tetrazolium dye MTT, which is reduced by living cells to form a formazan product that can be quantified spectrophotometrically.
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Lab products found in correlation
2 protocols using cell viability kit mtt
1
Cell Proliferation and Viability Assay
2
Hydrogel Synthesis and Characterization
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MMW chitosan (Sigma, code: 448877), HMW chitosan (Sigma, code: 419419), PVA (Sigma, code: P1763) used as polymers for hydrogel were purchased from Sigma-Aldrich (USA). rh-EGF (Sigma, code: E9644), metoksi PEG propionaldehyde (MW 5 kD, Sigma, JKA 3039), metoksi PEG propionaldehyde (MW 10 kD, Sigma, JKA 3033), were purchased Sigma Aldrich (USA). rm-EGF (Gibco, P01133) was obtained Gibco (New Zealand). Cell viability kit, (MTT), (Roche, 11465007001, Switzerland) were purchased from Roche. All materials used in these studies were in analytical grade.
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