Pei max reagent

HEK293T cells were cultured in DMEM medium supplemented with 10% fetal bovine serum. Gene knockdown experiments were performed using shRNA-expressing lentiviruses^{62 (link)}. Targeting sequences of shRNA were as shown below, 5′-GGAGACGTATATGTTGAAA-3′ (shListerin-1), 5′-GGTGCTGCGGAAACTTTCAAA-3′ (shListerin-2), 5′-GCCAGTTGCCGTCGTCGTTAAT-3′ (shZNF598-1), 5′-GCCCAGGACTACTACAGCGACTAT-3′ (sh ZNF598-2), 5′-GATGCAGACGTTGAAAAAATA-3′ (shASCC3-1), 5′-GTAATGCTACTAATCGAATTA-3′ (shASCC3-2). Transfection of plasmid DNA listed in Supplemenraly Data 3 was carried out using PEI-max reagent according to the manufacturer’s instruction (Cosmo Bio).

HEK293T cells were cultured in DMEM medium (Nacalai Tesque, Kyoto, Kyoto, Japan) supplemented with 10% Fetal Bovine Serum (Sigma-Aldrich, St. Louis, MO, USA) and Penicillin-Streptomycin (Fujifilm Wako pure chemical corporation, Osaka, Osaka, Japan). Plasmid transfection was performed using the PEI-MAX reagent (Cosmo Bio, Koto, Tokyo, Japan).

HEK293T (RCB2202) cells were provided by the RIKEN BRC through the National Bio-Resource Project of MEXT, Japan. They were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and penicillin/streptomycin (PS) (100 U/mL). 293FT cells (R700-07) were from Thermo Fisher and were grown in DMEM with 10% FBS, 1x Non-Essential Amino Acids Solution (Thermo Fisher 11140-050), and PS (100 U/mL). Plasmid transfection was performed using the PEI-MAX reagent (Cosmo Bio, Koto, Tokyo, Japan). Used cell lines in this study are listed in Supplementary Table 3.