Folin–Ciocialteau reagent, NADH, lactate dehydrogenase (LDH), pyridoxal phosphate (PLP), N-ethylmaleimide (NEM), bathophenanthrolinedisulfonic acid (BPDS), 2,4-dinitrofluorobenzene (DNFB), 1,4-dithio-bis-(2-nitrobenzoic acid) (DTT), acetonitrile, and crystal violet (N-hexamethylpararosaniline) were obtained from Sigma-Aldrich Corp. (St. Louis, MO, USA). Potassium cyanide (KCN) was obtained from Merck (Darmstadt, Germany), sodium 3-mercaptopyruvate, trifluoroacetic acid (TFA), and 2-mercaptoethanol from Flucka Chemie GmbH. Nε-methyl-l -lysine was purchased from Bachem (Bubendorf, Switzerland). Fetal bovine serum, trypsin, and penicillin/streptomycin were obtained from HyClone Laboratories (Utah, USA). The Cytotoxicity Detection Kit (LDH) was obtained from Roche Applied Science. All the other chemicals were of reagent grade and purchased from common commercial suppliers.
Diallyl trisulfide (DATS) was purchased from Cayman Chemical Company (Michigan, USA) and dissolved in dimethyl sulphoxide (DMSO; Sigma-Aldrich Corp., St. Louis, MO, USA), and then diluted with the medium Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone Laboratories, Utah, USA) to the desired concentration prior to its use (the final concentration of DMSO in the medium was less than 0.1%).
Diallyl trisulfide (DATS) was purchased from Cayman Chemical Company (Michigan, USA) and dissolved in dimethyl sulphoxide (DMSO; Sigma-Aldrich Corp., St. Louis, MO, USA), and then diluted with the medium Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone Laboratories, Utah, USA) to the desired concentration prior to its use (the final concentration of DMSO in the medium was less than 0.1%).