The contents of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in renal cortex were measured using the enzyme-linked immunosorbent assay (ELISA) kits (DG20049D and DG94490Q, Dogesce, Beijing, China). The levels or activities of Fe2+, lipid peroxide (LPO), superoxide anionic (O2-), malondialdehyde (MDA), total superoxide dismutase (T-SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) were measured using the colorimetric assay kits (E-BC-K773-M, E-BC-K176-M, E-BC-K001-M, E-BC-K025-M, E-BC-K020-M, E-BC-K030-M and E-BC-K096-M, Elabscience, Wuhan, China). All tests and analyses were performed according to the manufacturer’s instructions.
E bc k096 m
Manufactured by Elabscience
Sourced in United States
E-BC-K096-M is a laboratory equipment product from Elabscience. It is a device used for the measurement and analysis of various samples or substances. The core function of this product is to provide accurate and reliable data for scientific research and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
E bc k020 m, by Elabscience (3 mentions)
E bc k025 m, by Elabscience (2 mentions)
E bc k031 m, by Elabscience (2 mentions)
E bc k176 m, by Elabscience (1 mentions)
E bc k030 m, by Elabscience (1 mentions)
E bc k773 m, by Elabscience (1 mentions)
E el 0060c, by Elabscience (1 mentions)
E el h6008, by Elabscience (1 mentions)
E el h0149c, by Elabscience (1 mentions)
E el h0109c, by Elabscience (1 mentions)
E el h6156, by Elabscience (1 mentions)
3 protocols using e bc k096 m
1
Renal Oxidative Stress and Inflammation
2
Oxidative Stress and Inflammatory Biomarkers
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The activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) were measured using GSH-Px, CAT, and SOD activity assay kits (Catalog No.: E-BC-K096-M, E-BC-K031-M, E-BC-K020-M; Elabscience, Wuhan, China), respectively. Malondialdehyde (MDA), interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) levels were measured using MDA, IL-1β, IL-6, IL-8, and TNF-α ELISA kits (Catalog No.: E-EL-0060c, E-EL-H0149c, E-EL-H6156, E-EL-H6008, E-EL-H0109c, Elabscience). After cell culture at 24 h, the culture supernatant was collected and centrifuged at 1000 × g for 20 min to remove impurities and cellular debris. The supernatant was collected for further analysis. For the detection, 50 µl of the supernatant was taken and analyzed. Following completion of the experiment, the optical density (OD) of each well was measured using a Multiscan MK3 reader (Thermo Fisher Scientific). All reactions were repeated thrice.
3
Immunological and Oxidative Stress Assays
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ELISA kits for Interferon-γ (Catalog No: E-EL-R0009), Interleukin-2 (Catalog No: E-EL-R0013), Immunoglobulin type G (Catalog No: E-EL-R0518) and type M (Catalog No: E-EL-R3016) while colorimetric assay kits for malondialdehyde (MDA; E-BC-K025-M), total superoxide dismutase (T-SOD; E-BC-K020-M), glutathione peroxidase (GSH-Px; E-BC-K096-M) and catalase (CAT; E-BC-K031-M) were purchased from Elabscience Biotechnology, USA.
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