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Rabbit p27 kip1 antibody

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The Rabbit P27 Kip1 antibody is a primary antibody that binds to the p27 Kip1 protein, a cyclin-dependent kinase inhibitor involved in cell cycle regulation. This antibody can be used to detect and quantify the p27 Kip1 protein in various experimental applications.

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Lab products found in correlation

Ab110641, by Abcam (2 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (2 mentions) Emt antibody sampler kit, by Abcam (2 mentions) Rabbit cdk2 antibody, by Cell Signaling Technology (2 mentions) Rabbit p21 waf1 cip1 antibody, by Cell Signaling Technology (2 mentions) Rabbit akt antibody, by Cell Signaling Technology (2 mentions) Rabbit phospho aktser473 antibody, by Cell Signaling Technology (2 mentions) Rabbit phospho aktthr308 antibody, by Cell Signaling Technology (2 mentions) Mouse β actin antibody, by Proteintech (2 mentions) Ecl detection system, by Thermo Fisher Scientific (2 mentions) Sds page gel, by Bio-Rad (2 mentions) Kgp250, by Keygen Biotech (2 mentions)

2 protocols using rabbit p27 kip1 antibody

1

Protein Expression Analysis of PCa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RIPA lysis buffer containing protease inhibitors (# KGP250, KeyGEN BioTECH, Nanjing, China) was used to extract PCa cell protein following the standard protocol. Then, equal amounts of proteins in the cell lysates were separated by SDS/PAGE gels (4–12%, Bio-Rad) and electronically transferred onto polyvinylidene fluoride (PVDF, Millipore) membranes. The membranes were then blocked with 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with the following specific primary antibodies: rabbit CCL20 antibody (#ab9829, Abcam) and rabbit CCR6 antibody (#ab110641, Abcam); EMT Antibody Sampler Kit (#9782), rabbit CD68(#97778) and CD163(#93498), rabbit CDK2 antibody (#18048), rabbit P27 Kip1 antibody (#3686), rabbit p21 Waf1/Cip1 antibody (#2947), rabbit Akt antibody (#4691), rabbit phospho-AktSer473 antibody (#4060), and rabbit phospho-AktThr308 antibody (#13038) were purchased from Cell Signaling Technology except for mouse β-actin antibody (#60008–1-Ig, Proteintech Group). Subsequently, horseradish peroxidase (HRP)-conjugated secondary antibody was used to incubate the samples for 1 h at room temperature. The bands were visualized using the enhanced chemiluminescence (ECL) detection system (Pierce Biotechnology, Rockford, IL, United States).
Xie T., Fu D.J., Li Z.M., Lv D.J., Song X.L., Yu Y.Z., Wang C., Li K.J., Zhai B., Wu J., Feng N.H, & Zhao S.C. (2022). CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling. Molecular Cancer, 21, 173.
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2

Protein Expression Analysis of PCa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RIPA lysis buffer containing protease inhibitors (# KGP250, KeyGEN BioTECH, Nanjing, China) was used to extract PCa cell protein following the standard protocol. Then, equal amounts of proteins in the cell lysates were separated by SDS/PAGE gels (4–12%, Bio-Rad) and electronically transferred onto polyvinylidene fluoride (PVDF, Millipore) membranes. The membranes were then blocked with 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with the following specific primary antibodies: rabbit CCL20 antibody (#ab9829, Abcam) and rabbit CCR6 antibody (#ab110641, Abcam); EMT Antibody Sampler Kit (#9782), rabbit CD68(#97778) and CD163(#93498), rabbit CDK2 antibody (#18048), rabbit P27 Kip1 antibody (#3686), rabbit p21 Waf1/Cip1 antibody (#2947), rabbit Akt antibody (#4691), rabbit phospho-AktSer473 antibody (#4060), and rabbit phospho-AktThr308 antibody (#13038) were purchased from Cell Signaling Technology except for mouse β-actin antibody (#60008–1-Ig, Proteintech Group). Subsequently, horseradish peroxidase (HRP)-conjugated secondary antibody was used to incubate the samples for 1 h at room temperature. The bands were visualized using the enhanced chemiluminescence (ECL) detection system (Pierce Biotechnology, Rockford, IL, United States).
Xie T., Fu D.J., Li Z.M., Lv D.J., Song X.L., Yu Y.Z., Wang C., Li K.J., Zhai B., Wu J., Feng N.H, & Zhao S.C. (2022). CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling. Molecular Cancer, 21, 173.
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