Small interference RNA targeting DLX6-AS1 (si-DLX6-AS1: 5ʹ-AAUAAAGAACACUUACACUACUG-3ʹ) together with its negative control (si-NC: 5ʹ- UUCUCCGAACGUGUCACGUTT-3ʹ) were assembled by Ribobio (Guangzhou, China). miR-506-3p mimics (miR-506-3p; Product ID: miR10002878-1-5), miR-506-3p inhibitors (anti-miR-506-3p; Product ID: miR20002878-1-5) and respective controls, including miR-NC (Product ID: miR1N0000001-1-5) and anti-miR-NC (Product ID: miR2N0000001-1-5) were purchased from Ribobio. The sequences of DLX6-AS1 and STAT2 were amplified and cloned into the pcDNA3.1 overexpression vector, named as pcDNA-DLX6-AS1 and pcDNA-STAT2 (Sangon, Shanghai, China), and pcDNA empty vector was served as the negative control (pcDNA-NC). For stable DLX6-AS1 knockdown, a lentiviral vector containing short hairpin RNA targeting DLX6-AS1 (sh-DLX6-AS1: 5ʹ-GGTTCAGTATAGATTTCTA-3ʹ) and its negative control (sh-NC: 5ʹ- TTCTCCGAACGTGTCACGT-3ʹ) were synthesized by Research-Bio Co., Ltd (Shanghai, China). These oligonucleotides (20 nM) and plasmids (1 µg) were transfected into SK-N-SH and LAN-6 cells (5×104 cells) using Lipofectamine 3000 Reagent (Invitrogen, Carlsbad, CA, USA).
Anti mir 506 3p
Manufactured by RiboBio
Sourced in China
Anti-miR-506-3p is a laboratory reagent designed to target and inhibit the expression of miR-506-3p, a microRNA molecule. Its core function is to provide a tool for researchers to modulate the levels of miR-506-3p in their experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti mir nc, by RiboBio (3 mentions)
Si nc, by RiboBio (3 mentions)
Mir nc, by RiboBio (3 mentions)
Mir 506 3p, by RiboBio (2 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna dlx6 as1, by Sangon (1 mentions)
Pcdna nc, by Sangon (1 mentions)
Pcdna3.1 vector, by GenePharma (1 mentions)
Fugene 6, by Roche (1 mentions)
Pcdna, by RiboBio (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mir 506 3p
1
Targeting DLX6-AS1 and miR-506-3p in Neuroblastoma
2
Targeting Circular RNA circ_0003928 and miR-506-3p in HK-2 Cells
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The small interfering RNA against circ_0003928 (si-circ_0003928, 5′-AAGAGGATGGCCCCAGAAGTA-3′), the mimics of miR-506-3p (miR-506-3p, 5′-UAAGGCACCCUUCUGAGUAGA-3′), the inhibitors of miR-506-3p (anti-miR-506-3p, 5′-UCUACUCAGAAGGGUGCCUUA-3′), and respective controls, including siRNA negative control (si-NC), the negative control of miRNA mimics (miR-NC) and the negative control of miRNA inhibitors (anti-miR-NC) were synthesized in Ribobio Co., Ltd. (Guangzhou, China). The open reading frame of HDAC4 was amplified and then inserted into the pcDNA 3.1 vector by GenePharma Co., Ltd. (Shanghai, China) to generate the overexpression plasmid of HDAC4 (HDAC4). Then, cell transfection was performed on HK-2 cells in accordance with the manufacturer’s direction of FuGENE6 (Roche, Basel, Switzerland).
3
Modulating circUBAP2 and SEMA6D in CDDP-resistant Osteosarcoma
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Small interfering RNA (siRNA) against circUBAP2#1/2 (si-circUBAP2#1/2) and its negative control (si-NC), SEMA6D overexpression plasmid (SEMA6D) and its negative control (pcDNA), miR-506-3p mimic and inhibitor (miR-506-3p and anti-miR-506-3p) or their negative controls (miR-NC and anti-miR-NC) were synthesized by Ribobio (Guangzhou, China). All plasmid vectors were transfected into U2OS/CDDP and SaOS-2/CDDP cells using Lipofectamine 3000 (Invitrogen).
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