According to the manufacturer’s recommendations, we used the mouse ProtocartaPlex 10-plex immunoassay kit (Thermofisher Scientific, Vienna, Austria) to measure cytokine levels in serum samples. We classified the immune mediators into five categories: proinflammatory mediator (Monocyte Chemoattractant Protein [MCP]–1), type 1 cytokines (IL-2, interferon [IFN]- γ, and tumor necrosis factor [TNF]–α), type 2 cytokines (IL-4, IL-5, IL-10, and IL-13), a T helper 17 cytokine (IL-17), and a regulatory cytokine (Transforming Growth Factor [TGF]-β1). Data were acquired on the MAGPIX-Luminex XMAP technology (Merck Millipore, Darmstadt, Germany) using the Luminex XPONENT software solutions. The concentration (pg/mL) of each cytokine was obtained by interpolating MFI (median fluorescent intensity) of a dilution standard curve over seven dilution points supplied with the kit and calculated by the ProtocartaPlex Analyst 1.0 software (eBioscience, Thermo Fisher Scientific, USA).
Magpix luminex xmap technology
Manufactured by Merck Group
Sourced in Germany
The MAGPIX-Luminex XMAP technology is a multiplex assay platform that utilizes magnetic beads and flow cytometry to simultaneously detect and quantify multiple analytes in a single sample. It employs Luminex's proprietary XMAP technology to enable high-throughput, quantitative analysis of biomolecules. The core function of the MAGPIX system is to provide a flexible and efficient solution for multiplexed bioassays.
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2 protocols using magpix luminex xmap technology
1
Multiplex Cytokine Profiling in Mouse Serum
2
Multiplex Cytokine Profiling in Mice
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We measured cytokine levels in serum samples using the mouse ProtocartaPlex 10-plex immunoassay kit (Thermofisher Scientific, Vienna, Austria), following the manufacturer's recommendations. Data were acquired on the MAGPIX®-Luminex XMAP technology (Merck Millipore, Darmstadt, Germany) using the Luminex XPONENT software solutions. The concentration (pg/mL) of each cytokine was determined by interpolating the median fluorescent intensity (MFI) of a dilution standard curve over seven dilution points supplied with the kit and calculated by the ProtocartaPlex Analyst 1.0 software (eBioscience, Thermo Fisher Scientific, USA).
The immune mediators were classified into four categories: T helper-1 cytokines (IL-2, IFN-γ, and TNF-α), T helper-2 cytokines (IL-4, IL-5, and IL-13), T helper-17 cytokine (IL-17A), and regulatory cytokines (TGF-β1 and IL-10).
The immune mediators were classified into four categories: T helper-1 cytokines (IL-2, IFN-γ, and TNF-α), T helper-2 cytokines (IL-4, IL-5, and IL-13), T helper-17 cytokine (IL-17A), and regulatory cytokines (TGF-β1 and IL-10).
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