Hydrangea serrata (Thunb.) Ser. were cultivated in Yeongju, Korea and were identified by Dr. Hyung-Jun Kim from Forest Medicinal Resources Research Center in National Institute of Forest Science (NIFoS, Seoul, Korea). A specimen voucher has been deposited in COSMAXBIO (COSMAXBIO, Seongnam, Korea). The dried leaves of H. serrata (1000 g) were extracted with distilled water at 98 °C for 5 h followed by filtration and then spray-dried to give a dried extract residue WHS (261 g, 26.1%). The HPLC system consisted of a Waters model 2695 HPLC pump with photodiode array detector (Waters model 2998) set to 228 nm (Waters, Milford, MA, USA). Separations were achieved at 30 °C using a Luna C18 (4.6 mm, 250 mm, I.D., 5 μm, Phenomenex) column. The gradient mobile phases consisted of solvent A (100% acetonitrile) and solvent B (water) with a gradient elution as follows: 0 to 15 min, 20% to 25%; 15 to 30 min, 25% to 50%; 30 to 40 min, 50% to 100%; 40 to 50 min, 100% to 20% as percent of solvent A at a flow rate of 1.0 mL/min.
Waters model 2998
Manufactured by Waters Corporation
Sourced in United States
The Waters model 2998 is a photodiode array (PDA) detector. It is designed to detect and quantify multiple analytes simultaneously in high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) systems.
Automatically generated - may contain errors
Lab products found in correlation
Luna c18, by Phenomenex (1 mentions)
P 1010 polarimeter, by Jasco (1 mentions)
5 mm cptci cryoprobe, by Bruker (1 mentions)
Type 5554 silica gel plates, by Cytiva (1 mentions)
Mkc18f plates, by Cytiva (1 mentions)
1525 binary hplc pump, by Waters Corporation (1 mentions)
Av 600 spectrometer, by Bruker (1 mentions)
J 815 cd spectrometer, by Jasco (1 mentions)
2 protocols using waters model 2998
1
Hydrangea serrata Leaf Extraction Protocol
2
Characterization of Organic Compounds
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The 1 H and 13 C NMR spectra were recorded on a Bruker AV-600 spectrometer with a 5 mm CPTCI cryoprobe. 1 H chemical shifts are referenced to the residual DMSO-d 6 signal at H 2.49 and 13 C chemical shifts are referenced to the DMSO-d 6 solvent peak at C 39.5 ppm. Optical rotations were measured using a Jasco P-1010 Polarimeter with sodium light (589 nm). CD spectra were obtained on a Jasco J-815 CD Spectrometer. Low-and high-resolution MS were recorded using ESI ionization and a TOF mass analyzer. Merck Type 5554 silica gel plates and Whatman MKC18F plates were used for analytical thin layer chromatography. HPLC purifications were performed on a Waters 1525 binary HPLC pump attached to a photodiode array detector (PDA) Waters model 2998 scanning between max 200 and 400 nm. A C 18 reversed-phase InertSustain, 10 x 250 mm, 5 μm column manufactured in Japan was used for HPLC purifications. All solvents used were HPLC grade.
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