Total RNA was isolated from either kidney tissues or cultured cells using Tri reagent (Molecular Research Center, Inc, Cincinnati, OH, USA) per manufacturer's protocol. Single-strand cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) for two-step qRT-PCR. Quantitative PCR was performed using Taqman gene expression assays (TNF-α: Mm00443260_g1, CCL2/MCP-1: Mm00441242_m1, TNFR1: Mm00441883_g1, TNFR2: Mm00441889_m1, GAPDH: Mm99999915_g1; Life Technologies, Grand Island, NY, USA) in a Bio-Rad CFX96 Real-Time System. Data were analyzed using Bio-Rad CFX Manager Software version 2.0. Relative expression quantification was calculated using the 2(-ΔΔCT) equation after normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as described previously.15 (link),73
Tnfr2
Manufactured by Thermo Fisher Scientific
Sourced in United States
TNFR2 is a cell surface receptor that binds to the cytokine tumor necrosis factor (TNF). It plays a role in the regulation of immune responses and inflammation. The core function of TNFR2 is to mediate cellular signaling in response to TNF binding.
Automatically generated - may contain errors
Lab products found in correlation
Tnf α, by Thermo Fisher Scientific (2 mentions)
Cfx manager software version 2, by Bio-Rad (2 mentions)
Tnfr1, by Thermo Fisher Scientific (2 mentions)
Gapdh, by Thermo Fisher Scientific (2 mentions)
Tri reagent, by Molecular Research Center (2 mentions)
Iscript cdna synthesis kit, by Bio-Rad (2 mentions)
Cfx96 real time system, by Bio-Rad (2 mentions)
2 protocols using tnfr2
1
Gene Expression Analysis of Inflammatory Markers
2
Gene Expression Analysis of Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated from either kidney tissues or cultured cells using Tri reagent (Molecular Research Center, Inc, Cincinnati, OH, USA) per manufacturer's protocol. Single-strand cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) for two-step qRT-PCR. Quantitative PCR was performed using Taqman gene expression assays (TNF-α: Mm00443260_g1, CCL2/MCP-1: Mm00441242_m1, TNFR1: Mm00441883_g1, TNFR2: Mm00441889_m1, GAPDH: Mm99999915_g1; Life Technologies, Grand Island, NY, USA) in a Bio-Rad CFX96 Real-Time System. Data were analyzed using Bio-Rad CFX Manager Software version 2.0. Relative expression quantification was calculated using the 2(-ΔΔCT) equation after normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as described previously.15 (link),73
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