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Transstart top green qpcr suppermix kit

Manufactured by Transgene
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The TransStart Top Green qPCR SupperMix kit is a reagent used for quantitative real-time PCR (qPCR) analysis. It contains all the necessary components for performing qPCR reactions, including a DNA polymerase, dNTPs, and a green fluorescent dye for detection of amplified DNA.

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Lab products found in correlation

Transscript first strand cdna synthesis supermix kit, by Transgene (4 mentions) Cfxtm real time thermal cycler, by Bio-Rad (4 mentions) Trizol reagent, by Thermo Fisher Scientific (3 mentions) Trizol, by Thermo Fisher Scientific (1 mentions)

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TransStart Top Green qPCR Kit (2 citations)

4 protocols using transstart top green qpcr suppermix kit

1

RNA Extraction and qPCR Analysis

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Total RNA was extracted using TRIZOL (Invitrogen), and reverse transcription was performed using the TransScript First-Strand cDNA Synthesis SuperMix Kit (TransGen Biotech, Beijing, China) according to the manufacturer's recommendations. qPCR was performed using a CFXTM real-time thermal cycler (Bio-Rad, Hercules, CA, USA) using a TransStart Top Green qPCR SupperMix kit (TransGen Biotech). The primers used are listed in Table S2.
Gao R., Li D., Xun J., Zhou W., Li J., Wang J., Liu C., Li X., Shen W., Qiao H., Stupack D.G, & Luo N. (2018). CD44ICD promotes breast cancer stemness via PFKFB4-mediated glucose metabolism. Theranostics, 8(22), 6248-6262.
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2

RNA Extraction and qPCR Analysis

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Total RNA was extracted using TRIZOL reagent (Invitrogen) following manufacturer's instructions. cDNA was then synthesized using the Trans Script First-Strand cDNA Synthesis Super Mix Kit (Trans Gen Biotech, Beijing, China). qPCR was performed in a CFXTM real-time thermal cycler (Bio-Rad, Hercules, CA, USA) using a Trans Start Top Green qPCR Supper Mix kit (Trans Gen Biotech). Data analysis was performed with the comparative ΔCt method using GAPDH as internal control 13 (link). The sequences of the primers used in this study are listed in Table S3.
Li J., Xu J., Li L., Ianni A., Kumari P., Liu S., Sun P., Braun T., Tan X., Xiang R, & Yue S. (2020). MGAT3-mediated glycosylation of tetraspanin CD82 at asparagine 157 suppresses ovarian cancer metastasis by inhibiting the integrin signaling pathway. Theranostics, 10(14), 6467-6482.
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3

Comprehensive Transcriptomic Analysis Pipeline

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Total RNA was extracted using TRIZOL reagent (Invitrogen) following manufacturer’s instructions. cDNA was then synthesized using the Trans Script First-Strand cDNA Synthesis Super Mix Kit (Trans Gen Biotech, Beijing, China). qPCR was performed in a CFXTM real-time thermal cycler (Bio-Rad, Hercules, CA, USA) using a Trans Start Top Green qPCR Supper Mix kit (Trans Gen Biotech). Data analysis was performed with the comparative ΔCt method using GAPDH as internal control24 (link). The sequences of the primers used in this study are listed in Supplementary Table 2.
Total RNA was performed the sequencing on a BGISEQ-500 platform by BGI group. Raw reads were aligned to transcriptome using Bowtie2 (v.2.5.5) and RESM using a human hg38 reference from UCSC.
Liu S., Wang H., Li J., Zhang J., Wu J., Li Y., Piao Y., Pan L., Xiang R, & Yue S. (2020). FZR1 as a novel biomarker for breast cancer neoadjuvant chemotherapy prediction. Cell Death & Disease, 11(9), 804.
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4

Quantitative Gene Expression Analysis

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Total RNA was extracted using TRIZOL reagent (Invitrogen, Carlsbad, CA), and reverse transcribed into cDNA using the TransScript First-Strand cDNA Synthesis SuperMix Kit (TransGen Biotech, Beijing, China) according to manufacturer's recommendations. qPCR was performed on a CFXTM Real-Time Thermal cycler (Bio-Rad, Hercules, CA) using a TransStart Top Green qPCR SupperMix kit (TransGen Biotech, Beijing, China). The primers used were as follows: HAS2, 5'-TGACAGGCATCTCACGAACC-3', and 5'-GGGTCTGCTGGTTTAGCCAT-3'; GAPDH, 5'-CTCTGATTTGGTCGTATTGGG-3', and 5'-TGGAAGATGGTGATGGGATT-3'.
Gao R., Liu Y., Li D., Xun J., Zhou W., Wang P., Liu C., Li X., Shen W., Su W., Qiao H., Stupack D.G, & Luo N. (2018). PFKFB4 Promotes Breast Cancer Metastasis via Induction of Hyaluronan Production in a p38-Dependent Manner. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50(6).
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