Il 17 bv421

Manufactured by BioLegend

IL-17 BV421 is a fluorochrome-conjugated antibody used in flow cytometry applications. It specifically binds to the human IL-17 protein, which is a pro-inflammatory cytokine produced by Th17 cells. The BV421 fluorochrome enables detection in the violet laser/channel.

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Lab products found in correlation

Il 2 apc, by BioLegend (2 mentions) Golgiplug, by BD (2 mentions) Live dead aqua, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm buffer set, by BD (2 mentions) Cd8 apc cy7, by BioLegend (2 mentions) Ifn γ fitc, by BioLegend (2 mentions) Tnf bv605, by BioLegend (2 mentions) Attune nxt acoustic cytometer, by Thermo Fisher Scientific (2 mentions) Ifn γ pe, by BioLegend (1 mentions) Brefeldin a, by BioLegend (1 mentions) Mhcii, by BD (1 mentions) Ionomycin, by STEMCELL (1 mentions) Cytofastfix perm, by BioLegend (1 mentions) Cd4 af700, by BioLegend (1 mentions) Cd3 pe cy5, by BioLegend (1 mentions) Il 4 apc, by BioLegend (1 mentions) Granzyme b bv421, by BioLegend (1 mentions)

Close spelling variants of this product

IL-17 (65 citations) Anti-IL-17 BV421 (2 citations)

3 protocols using il 17 bv421

Pneumococcal Infection: T-cell Immune Profiling

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WT and Zip8-KO mice were infected with S. pneumoniae as previously described and mediastinal lymph nodes harvested 72 hours post infection. Lymph nodes were processed into single cell suspensions, stained with an antibody cocktail consisting of CD11c, MHC-II, CD24, CD64, CD80, CD86, CD40, CD45, CD3, CD8α and CD4 (BD Biosciences and BioLegend) and DC and T-cell populations were then phenotyped by flow cytometry.
To characterize CD4⁺ T-helper cell subsets, lymph node cell homogenates from WT and Zip8-KO infected mice were stimulated in vitro with a cell activation cocktail containing PMA (10 ng/ml; StemCell Technologies, Vancouver, Canada), ionomycin (250 ng/ml; StemCell Technologies) and Brefeldin A (5 μg/ml; BioLegend) for 4 hours. Cells were harvested, washed, stained for surface markers as previously described, fixed and permeabilized for 20 minutes with CytoFastFix/Perm (BioLegend), washed and then stained with an antibody cocktail containing IL-2 APC, IL-4 PE/Dazzle594, IFN-γ PE and IL-17 BV421 (BioLegend). Intracellular cytokine production of the CD4⁺ T-cell subset was characterized by flow cytometry.
For cytokine detection by ELISAs, LN leukocytes were stimulated with PMA (10 ng/ml) and ionomycin (250 ng/ml) for 48 hours. Supernatants were collected for measurement of IL-2, IFN-γ, IL-4 and IL-17A/F according to manufacturers’ instructions.

Hall S.C., Smith D.R., Dyavar S.R., Wyatt T.A., Samuelson D.R., Bailey K.L, & Knoell D.L. (2021). Critical Role of Zinc Transporter (Zip8) in Myeloid Innate Immune Cell Function and the Host Response against Bacterial Pneumonia. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1357-1370.

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Cytokine Production Profiling of Activated T Cells

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For functional assays on cytokine production by T cells, thawed isolated PBMCs were stimulated for 16 h at 37 °C in a 5% CO₂ atmosphere with anti-CD3/CD28 (1 μg/mL) in complete culture medium (RPMI 1640 supplemented with 10% fetal bovine serum and 1% each of l-glutamine, sodium pyruvate, nonessential amino acids, antibiotics, 0.1 M HEPES, 55 μM β-mercaptoethanol). For each sample, at least 2 million cells were left unstimulated as negative control, and 2 million cells were stimulated. All samples were incubated with a protein transport inhibitor containing brefeldin A (Golgi Plug, Becton Dickinson) and previously titrated concentration of CD107a-PE. After stimulation, cells were stained with LIVE-DEAD Aqua (ThermoFisher Scientific) and surface mAbs recognizing CD3 PE- Cy5, CD4 AF700, and CD8 APC-Cy7 (Biolegend, San Diego, CA, USA). Cells were washed with stain buffer, and fixed and permeabilized with the cytofix/cytoperm buffer set (Becton Dickinson) for cytokine detection. Cells were next stained with previously titrated mAbs recognizing IL-17 BV421, TNF BV605, IFN-γ FITC, IL-2 APC, or granzyme-B BV421 (all mAbs from Biolegend). Then, a minimum of 100,000 cells per sample were acquired on a Attune NxT acoustic cytometer (ThermoFisher).

De Biasi S., Meschiari M., Gibellini L., Bellinazzi C., Borella R., Fidanza L., Gozzi L., Iannone A., Lo Tartaro D., Mattioli M., Paolini A., Menozzi M., Milić J., Franceschi G., Fantini R., Tonelli R., Sita M., Sarti M., Trenti T., Brugioni L., Cicchetti L., Facchinetti F., Pietrangelo A., Clini E., Girardis M., Guaraldi G., Mussini C, & Cossarizza A. (2020). Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia. Nature Communications, 11, 3434.

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Cytokine Production Profiling of Activated T Cells

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For functional assays on cytokine production by T cells, thawed isolated PBMCs were stimulated for 16 h at 37 °C in a 5% CO2 atmosphere with anti-CD3/CD28 (1 μg/mL) in complete culture medium (RPMI 1640 supplemented with 10% fetal bovine serum and 1% each of l-glutamine, sodium pyruvate, nonessential amino acids, antibiotics, 0.1 M HEPES, and 55 μM β-mercaptoethanol). For each sample, at least 2 million cells were left unstimulated as negative control, and 2 million cells were stimulated. All samples were incubated with a protein-transport inhibitor containing brefeldin A (Golgi Plug, Becton Dickinson) and previously titrated concentration of CD107a-PE. After stimulation, cells were stained with LIVE-DEAD Aqua (ThermoFisher Scientific) and surface mAbs recognizing CD4 AF700, and CD8 APC-Cy7 (Biolegend, San Diego, CA, USA). Cells were washed with stain buffer and fixed and permeabilized with the cytofix/cytoperm buffer set (Becton Dickinson) for cytokine detection. Cells were next stained with previously titrated mAbs recognizing CD3 PE-Cy5, IL-17 BV421, TNF BV605, IFN-γ FITC, IL-4 APC, or granzyme-B BV421 (all mAbs from Biolegend). Then, a minimum of 100,000 cells per sample were acquired on a Attune NxT acoustic cytometer (ThermoFisher)^{53 (link)}. FCS data were acquired in list mode by Attune nxT Software v4.2. mAbs used are listed in Sup Data 6.

De Biasi S., Tartaro D.L., Gibellini L., Paolini A., Quong A., Petes C., Awong G., Douglas S., Lin D., Nieto J., Galassi F.M., Borella R., Fidanza L., Mattioli M., Leone C., Neri I., Meschiari M., Cicchetti L., Iannone A., Trenti T., Sarti M., Girardis M., Guaraldi G., Mussini C., Facchinetti F, & Cossarizza A. (2021). Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection. Nature Communications, 12, 4677.

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