For determination of sulfur content, roots and leaves samples were dried for 48 h at 65°C (Multiwave PRO, Anton Paar) and digested using 8 mL of concentrated HNO3 (65% Merck). Elements were analyzed by Inductively Coupled Plasma Optical Emission Spectrometry (iCAP 6500 dual OES spectrometer, Thermo Scientific, Waltham, MA, United States) by using Yttrium solution (1 ppm, Merck) as an internal standard.
Yttrium solution
Manufactured by Merck Group
Sourced in United States, Germany
Yttrium solution is a laboratory product that contains yttrium, a rare earth metal, dissolved in an appropriate solvent. It is used as a reference material or a starting material for various chemical processes and research applications.
Automatically generated - may contain errors
4 protocols using yttrium solution
1
Sulfur Content Determination in Plant Samples
2
Determination of Silicon Content
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For Si determination, 8 mL of 0.1 M Tiron solution buffered at pH 10.5 was added to 25 mg of DW, which was shaken continuously for 4 h at 65 °C in a shaker incubator (Infors HT, Minitron, Bottmingen, Switzerland). After cooling, 7 mL of H2O2 (Roth) was added in order to destroy Tiron. The tubes were shaken horizontally in a water bath maintained at 85 °C until the solution turned colorless. The samples were then centrifuged at 4000 rpm for 10 min at 25 °C before analysis. Elements were analyzed by Inductively Coupled Plasma Optical Emission Spectrometry (iCAP 6500 dual OES spectrometer, Thermo Scientific) using an Yttrium solution (1 ppm, Merck, Darmstadt, Germany) as an internal standard.
3
Elemental Analysis of Plant Leaves
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For determination of elements, fully expanded leaves were dried for 48 h at 65°C (Multiwave PRO, Anton Paar) and digested using 8 mL of concentrated HNO3 (65% Merck). For Si determination, 8 ml of 0.1 M Tiron solution buffered at pH 10.5 were added to 25 mg of DW which was continuously shaken for at least 4 h at 65°C in a shaker incubator (Infors HT, Minitron). After cooling, 7 mL of H2O2 (Roth) was added to destroy Tiron. The tubes were shaken horizontally in a water bath at 85°C until the solution turned colorless. The samples were then centrifuged at 4,000 rpm at 25°C for 10 min before analysis. The elements were analyzed by Inductively Coupled Plasma Optical Emission Spectrometry (iCAP 6500 dual OES spectrometer, Thermo Scientific) by using Yttrium solution (1 ppm, Merck) as an internal standard.
4
Trace Element Analysis in Tissues
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Trace elements can be analyzed using X-rays, generated by a molybdenum tube using the S2 Picofox™ (Bruker Nano GmbH, Berlin, Germany). Prior to measurement, mouse tissue samples were digested, whereas cell lysates were analyzed without further digestion. Briefly, 50 mg of tissue samples was weighted into microwave vessels and 830 µL ultrapure water, 900 µL 69% HNO3 (v/v) (Suprapure®, Merck/Millipore), 250 µL 30% H2O2 (v/v) (Sigma-Aldrich/Merck), and 20 µL of 200 mg/L yttrium-solution (Merck/Millipore) as digestion control were added. The samples were heated to 200 °C for 20 min using a Speedwave 2 (Berghof, Eningen, Germany). Afterwards, the samples were diluted with 2 mL ultrapure water and stored at RT. Certified reference material, namely pig kidney (ERM BB-186), was always analyzed in parallel. For the analysis of trace element content in mouse tissue and cells, 0.5 mg/L gallium (ThermoFisher Scientific) or 1 mg/L yttrium was used as internal standard. An amount of 10 µL of each sample was placed on a siliconized quartz glass carrier and dried overnight. Mouse and cell samples were measured randomly in triplicates for 1000 or 500 s, respectively.
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