Iron was introduced as ammonium ferric citrate (F5879, Sigma, St. Louis, MO, USA), dissolved in deionized water. Heme (H9039, Sigma, St. Louis, MO, USA) was dissolved in NaOH (20 mmol/L). Tin protoporphyrin IX (SnPP, 16375, Cayman Chemical, Ann Arbor, MI, USA) and zinc protoporphyrin IX (ZnPP, 691550-M, EMD Millipore Corp., Burlington, MA, USA) were dissolved in DMSO. The final concentration of NaOH was kept below 2 mmol/L, and the DMSO was less than 1% in all experiments. To deliver CO, we used the tricarbonyl-dichloro-ruthenium (II) dimer also known as CO-releasing molecule 2 (CORM2), [Ru2Cl4(CO)6] (288144, Sigma, St. Louis, MO, USA). CORM2 was dissolved in DMSO immediately before use, and it was administered every 12 h. The Nrf2 inhibitor ML385 (SML1833, Sigma, St. Louis, MO, USA) was dissolved in DMSO. Ferritin (FT) was administered as holoferritin (F4503, Sigma, St. Louis, MO, USA).
Tin protoporphyrin 9 snpp
Manufactured by Cayman Chemical
Sourced in United States
Tin protoporphyrin IX (SnPP) is a chemical compound that functions as a metalloporphyrin. It is a synthetic analogue of the natural heme group.
Automatically generated - may contain errors
Lab products found in correlation
Ferric ammonium citrate, by Merck Group (1 mentions)
Holo ferritin, by Merck Group (1 mentions)
Ml385, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Rotenone, by Merck Group (1 mentions)
Sulforaphane, by Enzo Life Sciences (1 mentions)
Brusatol, by Biosynth (1 mentions)
2 protocols using tin protoporphyrin 9 snpp
1
Administration of Iron, Heme, and Protoporphyrins
2
Cell Viability Examination Using CCK-8
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Cell viability was examined using Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan) as previously described [15] . Cells were seeded at a density of 5 × 10 3 cells per well in a 96-well plate. After 24 h, cells were treated with various concentrations of sulforaphane (Enzo Life Sciences Inc., Farmingdale, NY, USA) in the absence or presence of an HO-1 inhibitor (tin protoporphyrin IX, SnPP; Cayman Chemical, Ann Arbor, MI, USA), an Nrf2 signaling inhibitor (brusatol; Carbosynth Ltd., Newbury, Berkshire, UK), or a mitochondrial complex I inhibitor (rotenone; Sigma-Aldrich, St. Louis, MO, USA) for 24 h. The absorbance reflecting the number of living cells in each well was measured at 450 nm using a microplate reader (Sunrise ™ , Tecan Group Ltd., Männedorf, Switzerland).
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