Rabbit antibodies against IKKα (sc-7218, dilution 1:1,000), IKKβ (sc-7607, dilution 1:1,000), NEMO (sc-8330, dilution 1:1,000) and mouse antibody against ubiquitin (sc-8017, dilution 1:1,000) were obtained from Santa Cruz Biotechnology; antibodies against TAK1 (ab109526, dilution 1:1,000), UbcH7 (ab108936, dilution 1:2,000), Ubc13 (ab109286, dilution 1:5,000), RNF8 (ab131221, dilution 1:1,000), p-IKKα S176 (ab138426, dilution 1:500) were from Abcam; antibodies against CYLD (#8462, dilution 1:1,000), p-TAK1 Thr187 (#4536, dilution 1:1,000), p-IKKα/β Ser176/180 (#2697, dilution 1:1,000) and p-IκBα Ser32/36 (#9246, dilution 1:1,000) were from Cell Signaling; antibodies against FLAG-tag (M20008, dilution 1:5,000) and His-tag (M20001, dilution 1:1,000) were from Abmart; antibodies against IκBα and UbcH5c were homemade; and antibody against Tax (mAB, clone Lt-4) was described in Lee et al. [64 (link)].
P tak1 thr187
Manufactured by Cell Signaling Technology
P-TAK1 Thr187 is a lab equipment product that can be used to detect and measure the phosphorylation of TAK1 at threonine 187. It is a tool for researchers to study the activation and regulation of the TAK1 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
P iκbαser32 36, by Cell Signaling Technology (1 mentions)
His tag, by Abmart (1 mentions)
P ikkα β ser176 180, by Cell Signaling Technology (1 mentions)
Phosphatase inhibitor cocktail 3, by Merck Group (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Gapdh, by Bio-Rad (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Samhd1, by Abcam (1 mentions)
2 protocols using p tak1 thr187
1
Antibody Characterization for NF-κB Pathway
2
Comprehensive Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed in cell lysis buffer (Cell Signaling Technologies) containing protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail 3 (Sigma-Aldrich), 10 mM NaF, and 1 mM phenylmethylsulfonyl fluoride (PMSF). Cell lysates were analyzed by a bicinchoninic acid (BCA) assay (Pierce) for protein quantification, and equal amounts of protein were loaded for SDS-PAGE. Immunoblotting was performed as described using the following specific antibodies: HA (BioLegend; catalog no. 901501), IκBα (Abnova; catalog no. MAB0057), p-IκBα and TRAF6 (Cell Signaling Technology; catalog nos. 9246S and 8028, respectively), p-p38 and p38 MAPK (Cell Signaling Technology; catalog nos. 4511T and 8690T, respectively), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Bio-Rad; catalog no. AHP1628), SAMHD1 (Abcam, catalog no. 67820, or ProSci; catalog no. 8007), TAB3 (Cell Signaling Technology; catalog no. 14241S), TAK1 (Cell Signaling Technology; catalog no. 5206S) and pTAK1 (Thr187) (Cell Signaling Technology; catalog no. 4536S), and Flag (Sigma; catalog no. F1804). Detection of GAPDH protein was used as a loading control. For densitometry, low-exposure images were analyzed using ImageJ or Image Studio Lite, and the signal for the target protein was normalized to that of GAPDH (17 (link)).
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