Anti g6pc

Avicularin (purity > 99%) and lead acetate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The anti-p-IRE1, anti-PERK, anti-p-eIF2α, anti-GRP78, anti-PYG, anti-GK, anti-PEPCK, anti-G6PC, anti-TNF-α, anti-IL-1β and anti-β-actin antibodies were provided by Abcam (Cambridge, MA, USA and Santa Cruz Biotechnology, CA, USA) [5 (link)].

Protein samples (20 µg) of mouse liver were applied to 8% to 12% SDS-PAGE for electrophoretic separation and immunoblotting. The following were the primary antibodies used in the Western blot assay: anti-p-HSL (#4126; Cell Signaling Technology, Danvers, MA, USA), anti-HSL (#4107, Cell Signaling Technology), anti-PGC1α (#54481, Abcam, Cambridge, UK), anti-PPARγ (#7273, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-C/EBPα (#8178, Cell Signaling Technology), anti- PLIN2 (#108323, Abcam), anti-PCK1 (#70358, Abcam), anti-G6Pc (#83690, Abcam), anti-IRβ (#3025, Cell Signaling Technology), anti-IRS-1 (#3407, Cell Signaling Technology), anti-PI3K (#4257, Cell Signaling Technology), anti-p-AKT (#9271, Cell Signaling Technology), anti-AKT (#9272, Cell Signaling Technology), anti-CAT (#ER40125, HuaBio, Hangzhou, China), anti-SOD2 (#ET1701-54, HuaBio), anti-COX2 (#12282, Cell Signaling Technology), anti-GPX4 (#125066, Abcam), anti-FtH (#81444, Abcam), anti-xCT (#37185, Abcam), and anti-β-actin (#8457, Cell Signaling Technology). Peroxidase-conjugated goat anti-rabbit IgG (BL003A, Biosharp, Hefei, China) and goat anti-mouse IgG (BL001A, Biosharp) were applied as secondary antibodies.