Rabbit anti mst2

Embryonic lung tissues were homogenized in RIPA buffer supplemented with Complete Protease Inhibitor Cocktail tablets (Roche) and phosSTOP Phosphatase Inhibitor Cocktail tablets (Roche). The lysates were cleared and analyzed by Western blot as previously described (Lin et al., 2012 (link)).
The following primary antibodies were used: rabbit anti-YAP (Cell Signaling, 1:500), rabbit anti-phospho-YAP (Cell Signaling, 1:1000), rabbit anti-LATS (Cell Signaling, 1:1000), rabbit anti-phospho-LATS (Cell Signaling, 1:1000), rabbit anti-MST1 (Cell Signaling, 1:500), rabbit anti-MST2 (Cell Signaling, 1:1000), goat anti-MST1/2 (Santa Cruz 1:100), goat anti-CTGF (Santa Cruz, 1:200).

Protein lysate was prepared in SDS/Tris (pH7.6) lysis buffer, separated by electrophoresis in 8–10% SDS/PAGE gels, transferred to nitrocellulose membrane, and probed with the following antibodies: rabbit anti-YAP1 (4912; 1:1000), rabbit anti-p-Yap (Ser397) (13619; 1:1000), rabbit anti-GAPDH (2118; 1:1000), mouse anti-AMOT (60156–1-lg 1:500), rabbit anti-V5-tag (13202; 1:1000), rabbit anti-p65 (8242; 1:500), rabbit anti-p-p65 (Ser536) (3033; 1:500), rabbit anti-Caspase-3 (9662; 1:1000), rabbit anti-HSP90 (4875; 1:1000) (Cell Signaling Technology), rabbit USP31(12076–1-AP; 1:1000), rabbit anti-MYOD1 (18943–1-AP; 1:500) (Proteintech), rabbit FOXM1 (sc-502; 1:500) (Santa Cruz Biotechnology), c-Myc (32072; 1:2000), p57 Kip2 (75974; 1:500) (Abcam), mouse anti-Lamin B2 (D18) (University of Iowa Developmental Studies Hybridoma Bank). SuperSep Phos-tag™ gel was purchased from Wako. Rabbit anti-LATS1 (3477; 1:1000), rabbit anti-MST1 (3682; 1:1000), rabbit anti-MST2 (3952; 1:1000) (Cell Signaling Technology) were used to detect phosphorylated proteins.