Ici 182 780

Kp-10 (Metastin 45-54 (H-YNWNSFGLRF-NH 2 )) of human origin, a Kp form with a potency similar to Xenopus-Kp-10 for bullfrog Gpr54 (Moon et al. 2009) (link), was purchased from DBA Italia, Milan, Italy; Kp-234, a specific Gpr54 antagonist (Roseweir et al. 2009 (link)) was purchased from DBA Italia; 17B-estradiol (E 2 ) was purchased from Sigma-Aldrich S.R.L.; ICI 182780, a high-affinity estrogen receptor antagonist, was purchased from AstraZeneca, UK; and buserelin, a GnRH agonist, was a gift from Dr J Sandow, Hoechst, Frankfurt, Germany.
For Western blot and immunohistochemistry the following antibodies were used: goat polyclonal anti-Kiss-1 antibody (raised against a peptide mapping at the C-terminus of Kiss-1 of human origin; sc-18134) and rabbit polyclonal anti-Gpr54 antibody (raised against amino acids 141-342 mapping within an internal region of Gpr54 of human origin sharing 78% similarity with P. esculentus Gpr54; sc-134499); both were purchased from Santa Cruz Biotechnology and were validated for Western blot and immunohistochemistry (present data, Supplementary Figs 1 and2, see section on supplementary data given at the end of this article; Hsu et al. 2014 (link), Ciaramella et al. 2016) . Mouse monoclonal anti-α-tubulin (T6199) was purchased from Sigma-Aldrich.