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3 protocols using goat anti rabbit igg sc 2357

1

Quantifying Extracellular Matrix Proteins

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The antibodies against galectin-1(ab138513),and GAPDH(ab9485) were from Abcam (Cambridge, UK). The polyclonal antibodies against collagen I (bs-0578R), collagen III (bs-0549R), and fibronectin 1 (FN1)/Ugl-Y3 (bs-13455R) were from Bioss (Beijing, China). The goat anti-rabbit IgG (sc-2357) and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The MTT assay kit and dimethyl sulfoxide were from Sigma-Aldrich (St. Louis, MO, USA). The Modified Masson’s Trichrome Stain Kit was from Beijing Solarbio Science & Technology Co. Ltd. (G1346; Beijing, China).
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2

Molecular Mechanisms of GANT61-Mediated Inhibition

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GANT61 was purchased from Sigma Biotechnology (St. Louis, MO, USA). The anti- GAL-1 antibody was purchased from Cell Signaling Technology (13888S and 12936S, Danvers, MA, USA), the anti-GLI1 was purchased from Abcam (ab217326, Cambridge, UK) and Bioss (bs-1206R, Beijing, China), anti-MMP14 (ab78738), anti-MMP2(ab92536), anti-LAMC2 (ab210959 and ab274376) and anti-CD34(ab81289) antibodies were purchased from Abcam (Cambridge, UK), and the anti-GAPDH antibody(sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG(sc-516102) and goat anti-rabbit IgG(sc-2357) were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The PAS staining kit was provided by Leagene Biotechnology Co, Ltd (Beijing, People’s Republic of China). The MTT assay kit and dimethyl sulfoxide (DMSO) were provided by Sigma Biotechnology (St. Louis, MO, USA).
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3

Hedgehog Signaling Pathway Inhibitor ITD-1 Protocol

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ITD-1, a hedgehog signaling pathway inhibitor, was purchased from Macklin (1884570, Shanghai, China). The anti-GAL-1 antibody was purchased from Abcam (ab138513, Cambridge, UK), the anti-SMA-α antibody was purchased from Cell Signaling Technology (56856, Danvers, MA, USA), anti-E-cadherin (bs-10009R), anti-Vimentin (bs-0756R), anti-TGF-β1 (bs-0086R), and anti-p-Smad2/3(bs-8853R) antibodies were purchased from Bioss (Beijing, China), and the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody (sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102), and goat anti-rabbit IgG (sc-2357) antibodies were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The dimethyl sulfoxide (DMSO) and MTT assay kit and were provided by Sigma Biotechnology (St. Louis, MO, USA).
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