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Alexafluor488 conjugated anti chicken

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AlexaFluor488‐conjugated anti‐chicken is a fluorescently labeled antibody that binds to chicken-derived proteins. It is designed for use in various immunological techniques, such as flow cytometry, immunofluorescence, and Western blotting.

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Lab products found in correlation

Alexa fluor 594 conjugated anti rabbit, by Thermo Fisher Scientific (2 mentions) Inverted a1r spectral, by Nikon (2 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho histone3 ph3 antibodies, by Abcam (1 mentions) Gtx127342, by GeneTex (1 mentions)

Spelling variants of this product

Anti-AlexaFluor488 (4 citations) AlexaFluor488-conjugated anti-chicken secondary antibody (2 citations) AlexaFluor488-conjugated anti-chicken-IgG (2 citations)

2 protocols using alexafluor488 conjugated anti chicken

1

Fluorescent Embryo Imaging with IF Staining

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Transgenic fluorescent embryos were embedded in 1% agarose in a glass‐bottom dish. Immunofluorescence double staining was performed with chicken‐anti‐GFP (1:400; Life Technologies) and rabbit‐anti‐phospho‐histone3 (pH3) antibodies (1:250; Abcam). We used AlexaFluor488‐conjugated anti‐chicken (1:1,000; Life Technologies) and AlexaFluor594‐conjugated anti‐rabbit (1:1,000; Life Technologies) secondary antibodies to reveal primary antibodies. Confocal imaging was performed using a Nikon inverted A1r spectral.
Cacialli P., Mailhe M., Wagner I., Merkler D., Golub R, & Bertrand J.Y. (2022). Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates. The EMBO Journal, 41(19), e108536.
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2

Imaging DNA Damage in Transgenic Embryos

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Transgenic fluorescent embryos were embedded in 1% agarose dissolved in 1xE3 in a glass-bottom dish. For immunofluorescence, double staining was performed using chicken-anti-GFP (1:500; Life Technologies), rabbit-anti-H2A.X (phospho Ser139) (1:500; GTX127342; Genetex) rabbit, and pH3 (1:250; Abcam) antibodies. We used AlexaFluor488-conjugated anti-chicken (1:1,000; Life Technologies) and AlexaFluor594-conjugated anti-rabbit (1:1,000; Life Technologies) secondary antibodies to reveal primary antibodies. Confocal imaging was performed using a Nikon inverted A1r spectral.
Cacialli P., Dogan S., Linnerz T., Pasche C, & Bertrand J.Y. (2023). Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo. Stem Cell Reports, 18(7), 1534-1546.
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