Ab213511

Membranes were probed with antibodies against RIPK2 (1:1000, Cell Signaling Technology (CST) #4142 or Santa Cruz Biotechnology #sc-166765), phospho-NF-κB p65 (Ser536) (1;1000, CST #3033), NF-κB p65 (1:1000, CST #8242), IκBα (1:1000, CST #4814), c-Myc (1:5000, Abcam #ab32072), phospho-c-Myc (S62) (1:1000, CST #13748S), ubiquitin (K48-linkage specific) (1:1000, CST #12805), FLAG (1:5000, Sigma #F1804), MKK7 (1:1000, CST #4172S or 1:2000, Santa Cruz #sc-25288), MKK7 (phospho-Ser271) (1:1000, Aviva Systems Biology #OAAF05547), JNK (1:1000, CST #9252S or 1:2000 Santa Cruz sc-7345), JNK (phospho-T183/Y185) (1:1000, CST #9251S), mCherry (1:1000, Abcam #ab213511), GFP (1:1000, Abcam #ab290), β-actin (1:5000, Sigma Aldrich #5441), or GAPDH (1:1000, CST #3683). Signal was visualized with secondary HRP-conjugated antibodies (1:5000, CST #7074S or #7076S) and chemiluminescent detection.

Paraffin-embedded tissue blocks were cut into 2.5-μm sections and transferred to glass slides. Sections were immersed in 3% hydrogen peroxide and incubated with primary antibodies overnight at 4 °C. Subsequently, sections were stained with goat anti-mouse and anti-rabbit IgG/Alexa Fluor (Bioss Antibodies). The following primary antibodies were used: HAO1 (Immunoway, YM3378, 1:200 dilution), SP-C (Proteintech, 10774-1-AP, 1:200 dilution), mCherry (Abcam, ab213511, 1:500 dilution), citrullinated histone H3 (Abcam, ab5103, 1:200 dilution), neutrophil elastase (Abcam, ab131260, 1:200 dilution).