We used 5-month-old naïve OM and S5B rats. Slides with mounted brain sections at the level of dorsal striatum that were stored at −80°C were gradually brought back to room temperature and then exposed to receptor autoradiography procedures using [3H]spiperone, [3H]SCH23390, [3H]MPEP, and [3H]SR141716A as previously described21 (link), 26 (link)–28 (link). Radioactivity concentrations were verified via liquid scintillation counting. For [3H]spiperone and [3H]SCH23390 experiments, slides were placed in BAS-TR 2025 (GE Healthcare, Piscataway, NJ) phosphor imaging plates for 7 days along with [14C] standards (GE Healthcare, Piscataway, NJ) (calibrated against 3H-impregnated brain paste standards). Imaging plates were developed using an FLA-7000 phosphorimager (GE Healthcare, Piscataway NJ). Using Multigauge® software (GE Healthcare, Piscataway, NJ), regions of interest (ROIs) were drawn on the dorsolateral (DLST) and dorsomedial striatum (DMST) of each section. Values were averaged and initially expressed as PSL/mm2 and subsequently converted to dpm/mg with the use of [14C] standards. For [3H]MPEP and [3H]SR141716A experiments, slides were imaged using a β-imager (Biospace Lab, France). Scanning and image analysis methods were performed as previously described21 (link).
14c standards
Manufactured by GE Healthcare
[14C] standards are radioactive reference materials used to calibrate and verify the performance of radiation detection equipment. They provide a consistent and reliable source of radioactivity for laboratory testing and quality control purposes.
Automatically generated - may contain errors
2 protocols using 14c standards
1
Quantification of Neurotransmitter Receptors
2
Quantification of Neurotransmitter Receptors
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We used 5-month-old naïve OM and S5B rats. Slides with mounted brain sections at the level of dorsal striatum that were stored at −80°C were gradually brought back to room temperature and then exposed to receptor autoradiography procedures using [3H]spiperone, [3H]SCH23390, [3H]MPEP, and [3H]SR141716A as previously described21 (link), 26 (link)–28 (link). Radioactivity concentrations were verified via liquid scintillation counting. For [3H]spiperone and [3H]SCH23390 experiments, slides were placed in BAS-TR 2025 (GE Healthcare, Piscataway, NJ) phosphor imaging plates for 7 days along with [14C] standards (GE Healthcare, Piscataway, NJ) (calibrated against 3H-impregnated brain paste standards). Imaging plates were developed using an FLA-7000 phosphorimager (GE Healthcare, Piscataway NJ). Using Multigauge® software (GE Healthcare, Piscataway, NJ), regions of interest (ROIs) were drawn on the dorsolateral (DLST) and dorsomedial striatum (DMST) of each section. Values were averaged and initially expressed as PSL/mm2 and subsequently converted to dpm/mg with the use of [14C] standards. For [3H]MPEP and [3H]SR141716A experiments, slides were imaged using a β-imager (Biospace Lab, France). Scanning and image analysis methods were performed as previously described21 (link).
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