Metformin (1,1-Dimethylbiguanide hydrochloride, #D150959-5G), Protoporphyrin IX zinc (ZnPPIX, #282820), N-Acetyl-L-cysteine (NAC, #A7250) and 2',7'-Dichlorofluorescein diacetate (DCFH-DA, #D6883) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cycloheximide (CHX, CAS 66-81-9) was purchased from MedChemExpress LLC (Monmouth Junction, NJ, USA). Cisplatin and proteasome inhibitor MG132 was purchased from Selleck Chemicals (Houston, TX, USA). The Annexin V-PE/7-AAD Apoptosis Detection Kit (AP104) was purchased from Multi Sciences (Hangzhou, China). U0126 (#9903). The antibodies used for immunoblotting and co-immunoprecipitation, β-actin (#58169), Nrf2 (#12721), caspase-3 (#9662), poly ADP-ribose polymerase (PARP) (#9532), Phospho-c-Raf (#9431), p44/42 MAPK (Erk1/2, #4695), Phospho-p44/42 MAPK (#8544), and ubiquitin (#3936), along with goat anti-rabbit immunoglobulin G (IgG, #7074) and goat anti-mouse (#7076) HRP-linked secondary antibodies, were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Antibodies against Keap1 (AF5266) and HO-1 (AF5393) were obtained from Affinity Biosciences (Cincinnati, OH, USA).
Protoporphyrin 9 zinc znppix
Manufactured by Merck Group
Sourced in United States
Protoporphyrin IX zinc (ZnPPIX) is a laboratory reagent used in various research and analytical applications. It is a metal-containing compound that serves as a cofactor or substrate in certain enzymatic reactions and biological processes. The core function of ZnPPIX is to provide a source of zinc ions and serve as a model compound for studying zinc-related biochemical mechanisms. It is commonly used in research settings to investigate the role of zinc in cellular processes and the effects of zinc homeostasis.
Automatically generated - may contain errors
Lab products found in correlation
N acetyl l cysteine nac, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Metformin 1 1 dimethylbiguanide hydrochloride, by Merck Group (1 mentions)
P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
Phospho c raf, by Cell Signaling Technology (1 mentions)
Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions)
Poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ubiquitin, by Cell Signaling Technology (1 mentions)
Annexin 5 pe 7 aad apoptosis detection kit, by MultiSciences Biotech (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
U937 mononuclear cells, by American Type Culture Collection (1 mentions)
2 protocols using protoporphyrin 9 zinc znppix
1
Metformin, Protoporphyrin IX, and NAC Protocol
2
Astaxanthin Modulates U937 Mononuclear Cell Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
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U937 mononuclear cells were purchased from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in a 5% CO2 atmosphere in RPMI 1640 medium (GIBCO, Invitrogen) containing 10% fetal calf serum, 100 ng/mL streptomycin, 100 U/mL penicillin and 2 mM L-glutamine. Cells derived from the same freeze-down batch were thawed, grown and seeded (at 2×105 cells per well) onto six-well tissue culture plates and cultured in medium with and without 10 µM astaxanthin (ASTA) (Sigma-Aldrich, St. Louis, MO, USA), LPS (10 µg/mL) and 10 µM of Protoporphyrin IX zinc (ZnPP IX)(Sigma-Aldrich, St. Louis, MO, USA). ASTA was dissolved in dimethyl sulfoxide (DMSO) and diluted with the medium. The final concentration of DMSO in the medium was 0.5%. Control cells did not contain ASTA and/or LPS.
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