MPO activity assay was performed as described previously [24 (link)]. In brief, 10 μl brain protein of which had diluted as 1:10 from ipsilateral hemisphere was added to 180 μl of work solution containing 2 mmol/l O-dianisidin-dihydrochloride (Sigma) dissolved in 50 mmol/l PBS (pH = 6). Before measurement, 10 μl of 100 mmol/l H2O2 was added. Changes in absorbance at 460 nm over 10 min were measured.
O dianisidin dihydrochloride
Manufactured by Merck Group
O-dianisidin-dihydrochloride is a chemical compound used in laboratory settings. It is a dye precursor commonly employed in analytical and diagnostic procedures.
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2 protocols using o dianisidin dihydrochloride
1
Measuring MPO Activity in Brain Samples
2
Myeloperoxidase Activity Assay in Brain
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MPO activity assay was performed as described previously [23 (link)]. In brief, brain protein (10 μL) from ipsilateral hemisphere was added to 180 μL of work solution, which contained 2 mmol/L O-dianisidin-dihydrochloride (Sigma) dissolved in 180 μl of 50 mmol/L potassium phosphate buffer (pH =6). Before measurement, 10 μL of 100 mmol/L H2O2 was added. Changes in absorbance at 460 nm over 10 minutes were measured. MPO activity was expressed as U/mg tissue, and 1U of MPO activity represents the amount of enzyme degrading 1 μmol H2O2 per minute at 25°C.
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