Amersham typhoon rgb

Manufactured by Cytiva

Sourced in United States

The Amersham Typhoon RGB is a versatile phosphor and fluorescence imager designed for life science research applications. It offers high-resolution imaging and quantitative analysis capabilities for a wide range of samples, including gels, blots, and microarrays. The device utilizes multiple excitation lasers and sensitive detectors to capture high-quality images of various fluorescent and phosphor-labeled biomolecules.

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Lab products found in correlation

Imagequant, by Cytiva (1 mentions) Cuga 7 in vitro transcription kit, by Nippon Gene (1 mentions) Xcell surelock mini cell gel tank, by Thermo Fisher Scientific (1 mentions) Nativepage 4 16 bis tris gel, by Thermo Fisher Scientific (1 mentions) Nupage mes sds running buffer, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Amersham Typhoon RGB imager Amersham Typhoon RGB Biomolecular Imager Typhoon RGB Amersham Typhoon

3 protocols using amersham typhoon rgb

In Vitro Protein Synthesis Assay

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RNA templates produced by CUGA®7 in vitro transcription kit (Nippon Gene). The PURE system (PUREfrex, Gene-Frontier) reaction was carried out at 37 °C for 2 h in the presence or absence of 1 µM IbpA, which included Cy5 labeled tRNA^fMet. After protein synthesis, SDS-sample buffer (0.125 M Tris-HCl (pH 6.8), 10% (v/v) 2-mercaptoethanol, 4% (w/v) SDS, 10% (w/v) sucrose, 0.01% (w/v) bromophenol blue) was added and incubated at 95 °C for 5 min. The samples were then separated by SDS-PAGE and detected using a fluorescence imager (Amersham Typhoon RGB, Cytiva) at the 633-nm wavelength. The band intensity was quantified with image analysis software (Image quant, Cytiva).

Miwa T, & Taguchi H. (2023). Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of σ32. Proceedings of the National Academy of Sciences of the United States of America, 120(32), e2304841120.

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Titration of Transcription Factor H238A

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For FAM-labeled substrates, the final substrate concentration in the reaction was 20 nM. The concentration of H238A in the titration series varied across the gel lanes from 0 µM, 0.027 µM, 0.054 µM, 0.109 µM, 0.217 µM, 0.434 µM, and 0.868 µM in each lane, respectively. Binding reactions were incubated at room temperature in the binding buffer consisting of 30 mM HEPES (pH 7.5) and 100 mM potassium acetate. Samples was analyzed by 8% TBE native PAGE, run at 4 °C for 45 min at 10 V/cm and imaged on an Amersham Typhoon RGB (Cytiva).

Liu C., Hauk G., Yan Q, & Berger J.M. (2024). Structure of Escherichia coli exonuclease VII. Proceedings of the National Academy of Sciences of the United States of America, 121(5), e2319644121.

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Multimodal Protein Aggregation Analysis

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A combination of SDS-PAGE and Native-PAGE was used to analyze the aggregation states of the samples. The gels ran on the XCell Surelock Mini-Cell gel tank (Thermo Fisher Scientific), with different running buffers for SDS-PAGE and Native-PAGE.
For SDS-PAGE, total and soluble fractions were prepared and analyzed separately. To prepare the soluble fraction, 50 μL of the sample was centrifuged at 16,900 xg for 5 minutes and the supernatant was used as the soluble fraction. Three μL of Fluorescent Compatible Sample Loading Buffer (Thermo Fisher Scientific) was added to 9 μL of each sample and heated at 95 °C for 15 minutes. Samples were loaded onto a NuPAGE 4-12% Bis-Tris Gel and run at 200 V with NuPAGE MES SDS Running Buffer for 25 minutes (Thermo Fisher Scientific).
For Native-PAGE, 9 μL of the samples were loaded into each well of the Native-PAGE 4-16% Bis-Tris Gel (Thermo Fisher Scientific). Electrophoresis was performed at 150 V with Novex Tris-Glycine Native running buffer for 90 minutes.
Samples containing HiLyte Fluor 488 labeled Aβ42 (green) and Alexa Fluor 647 labeled αS (red), were utilized for fluorescence imaging of SDS- and Native-PAGE gels. The gels were imaged on an Amersham Typhoon RGB (Cytiva, Marlborough, MA, USA) located at the Genomics Core at NYU Center for Genomics and Systems Biology.

Chau E, & Kim J.R. (2022). α-synuclein-assisted oligomerization of β-amyloid (1-42). Archives of biochemistry and biophysics, 717, 109120.

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