All the reagents were used as received unless noted otherwise. Hexadecyltrimethylammonium bromide (CTAB, 98%) and Irgacure 2959 were purchased from Sigma-Aldrich. Chlorhexidine, Tetraethyloxysilane (TEOS), and gelatin methacryloyl (GelMA) were obtained from Aladdin Reagent Co. Ltd. Sodium hydroxide (NaOH), acetone and methanol (MeOH) were obtained from Sinopharm Chemical Reagent Limited Corporation and used as received. Trypsin-EDTA (0.25%), fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM), Hank’s Balanced Salt Solution, LIVE/DEAD™ Viability/Cytotoxicity Kit, LIVE/DEADTM BacLightTM Bacterial Viability Kit, and CCK-8 kit were purchased from Life Technologies. Trypticase soy broth (TSB), β-cyclodextrin (β-CD), and chlorhexidine were purchased from Solarbio. Hyaluronic acid (HA, MW = 30–50 kDa) was purchased from Bloomage Biotechnology Corporation Limited. All glassware and Teflon-coated magnetic stirring bars were carefully cleaned with aqua regia, followed by profuse washing with pure DI water using a Millipore system.
Trypticase soy broth (tsb)
Manufactured by Solarbio
Sourced in China
Trypticase Soy Broth (TSB) is a general-purpose, nutrient-rich culture medium used for the growth and maintenance of a wide variety of microorganisms. It provides the necessary nutrients and optimal growth conditions for a broad spectrum of bacteria, yeasts, and fungi.
Automatically generated - may contain errors
Lab products found in correlation
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Live deadtm baclighttm bacterial viability kit, by Thermo Fisher Scientific (1 mentions)
Irgacure 2959, by Merck Group (1 mentions)
β cyclodextrin βcd, by Solarbio (1 mentions)
Cck 8 kit, by Thermo Fisher Scientific (1 mentions)
Methanol meoh, by Sinopharm (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
S aureus atcc 43300, by American Type Culture Collection (1 mentions)
E coli atcc35218, by American Type Culture Collection (1 mentions)
2 protocols using trypticase soy broth (tsb)
1
Gelatin-based Antimicrobial Hydrogel Synthesis
2
Cell and Bacterial Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MC3T3-E1 osteoblast precursor cells and RAW264.7 macrophages were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Science. S. aureus (ATCC43300) and E. coli (ATCC35218) were obtained from the American Type Culture Collection. The cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum (Gibco). Bacteria were grown in trypticase soy broth (TSB, Solarbio, China). The cells and bacteria were passaged every 1 to 2 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!