Rabbit anti vps34

Manufactured by Cell Signaling Technology

Rabbit anti-VPS34 is a primary antibody that specifically recognizes the VPS34 protein. VPS34 is a class III phosphoinositide 3-kinase that plays a crucial role in the regulation of various cellular processes, including autophagy, endocytosis, and membrane trafficking.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using rabbit anti vps34

Comprehensive Antibody Panel for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were employed for either immunofluorescence or western blotting: mouse anti-ARH-I (for IF 1:250, for WB 1:1000; cod. ab45768; Abcam, Cambridge, UK), mouse anti-p21 (for IF 1:100, for WB 1:200; cod. sc-817; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-LC3 (1:2000; cod. L7543; Sigma-Aldrich), rabbit anti-p62 (1:500; cod. 8025; Cell Signaling, Danvers, MA, USA), mouse anti-β-actin (1:2000; cod. A5441; Sigma-Aldrich), mouse anti-BECLIN-1 (1:500; cod. 612112; BD Biosciences, Franklin Lakes, NJ, USA), goat anti-BECLIN-1 (1:250; cod. sc-10086; Santa Cruz Biotechnology), rabbit anti-VPS34 (1:500; cod. 4263; Cell signaling), mouse anti-STAT3 (1:500; cod. 9139; Cell Signaling), rabbit anti-phospho (Tyr705) STAT3 (1:1000; cod. 9145; Cell Signaling), rabbit anti-Ki-67 (1:100; cod. HPA001164; Sigma-Aldrich), mouse anti-BCL-2 (1:500; cod. 15071; Cell Signaling), mouse anti-IL-6R (1:1000; cod. AHR0061; Invitrogen, Waltham, MA, USA), rabbit anti-cyclin D1 (1:500; cod. 2978; Cell Signaling), rabbit anti-p38 (1:500; cod. 9212; Cell Signaling), rabbit anti-GAPDH (1:1000, cod. G9545, Sigma Aldrich), and mouse anti-ERK1/2 (1:500; cod. 05-1152; Millipore, Burlington, MA, USA).

Esposito A., Ferraresi A., Salwa A., Vidoni C., Dhanasekaran D.N, & Isidoro C. (2022). Resveratrol Contrasts IL-6 Pro-Growth Effects and Promotes Autophagy-Mediated Cancer Cell Dormancy in 3D Ovarian Cancer: Role of miR-1305 and of Its Target ARH-I. Cancers, 14(9), 2142.

+ Open protocol

+ Expand

Molecular Cloning and Tagging Strategies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human Dpr1 cDNA was cloned into pEGFP-C3 to generate GFP-Dpr1. Human Vps34 cDNA was cloned into the KpnI-XbaI sites of the pcDNA3.1(+)-HA or pCS2(+)-Flag vector. The Flag-Beclin1 was a gift from Dr Honggang Wang. The p62-GFP was a gift from Dr Terje Johansen. Dvl2 was cloned into the Bam HI site of pDsRed-N1. Atg14L was cloned from Atg14L-GFP into EcoRI-SalI sites of pmCherry-N1. Dpr1 shRNAs were purchased from Open Biosystems. MG132 was from Calbiochem and prepared as 100 mg/ml in DMSO. BafA1, rapamycin, and XAV939 were obtained from Sigma. A769662 was from Abcam. Antibodies include: mouse anti-Calbindin (Sigma), rabbit anti-GFAP (Bioss, Beijing), mouse anti-ubiquitin (FK2 MBL), mouse anti-Beclin1 (BD Biosciences), rabbit anti-Beclin1 (MBL), rabbit anti-Vps34 (Cell Signaling), rabbit anti-Atg14L (MBL), mouse anti-Flag M2 (Sigma), rabbit anti-Dvl2 (Cell Signaling), mouse anti-tubulin and anti-GFP (Santa Cruz Biotech), mouse anti-p62 and rabbit anti-LC3 (MBL).

Ma B., Cao W., Li W., Gao C., Qi Z., Zhao Y., Du J., Xue H., Peng J., Wen J., Chen H., Ning Y., Huang L., Zhang H., Gao X., Yu L, & Chen Y.G. (2014). Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation. Cell Research, 24(8), 912-924.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!