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Lentiviral vector encoding flag tagged cpt1b

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The Lentiviral vector encoding Flag-tagged CPT1B is a laboratory tool designed for gene expression studies. It contains the coding sequence for the Flag-tagged carnitine palmitoyltransferase 1B (CPT1B) protein, which is a key enzyme involved in fatty acid metabolism. This vector can be used to express the Flag-tagged CPT1B protein in cell lines or other experimental systems.

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Lab products found in correlation

Q5 mutagenesis kit, by New England Biolabs (2 mentions) Pspax2, by Addgene (2 mentions) Pmd2 g, by Addgene (2 mentions)

2 protocols using lentiviral vector encoding flag tagged cpt1b

1

Lentiviral Vectors for CPT1B Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral vector encoding Flag-tagged CPT1B was purchased from Origene. Site-directed mutagenesis was achieved using Q5 mutagenesis kit (New England Biolabs), by using the respective primers. Then, resulting lentiviral plasmids encoding else full-length Flag-CPT1B-WT, shorter Flag-CPT1B (CPT1B1–616, CPT1B1–350, CPT1B1–219), or Flag-CPT1B-P295A, or Flag-CPT1B-P295R, or plasmid encoding FLAG-VDAC1 or carboxy-terminal truncated VDAC1 (VDAC1-ΔC) were co-transfected as needed with lentiviral packaging plasmids psPAX2 (#12260, Addgene) and pMD2.G (#12259, Addgene) in HEK293T cells to ensure lentiviral particles production.
Angelini A., Saha P.K., Jain A., Jung S.Y., Mynatt R.L., Pi X, & Xie L. (2021). PHDs/CPT1B/VDAC1 axis regulates long-chain fatty acid oxidation in cardiomyocytes. Cell reports, 37(1), 109767.
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2

Lentiviral Vectors for CPT1B Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral vector encoding Flag-tagged CPT1B was purchased from Origene. Site-directed mutagenesis was achieved using Q5 mutagenesis kit (New England Biolabs), by using the respective primers. Then, resulting lentiviral plasmids encoding else full-length Flag-CPT1B-WT, shorter Flag-CPT1B (CPT1B1–616, CPT1B1–350, CPT1B1–219), or Flag-CPT1B-P295A, or Flag-CPT1B-P295R, or plasmid encoding FLAG-VDAC1 or carboxy-terminal truncated VDAC1 (VDAC1-ΔC) were co-transfected as needed with lentiviral packaging plasmids psPAX2 (#12260, Addgene) and pMD2.G (#12259, Addgene) in HEK293T cells to ensure lentiviral particles production.
Angelini A., Saha P.K., Jain A., Jung S.Y., Mynatt R.L., Pi X, & Xie L. (2021). PHDs/CPT1B/VDAC1 axis regulates long-chain fatty acid oxidation in cardiomyocytes. Cell reports, 37(1), 109767.
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