Thrombin from human plasma and apo-transferrin were purchased from Sigma-Aldrich (USA). BSA and fibrinogen from human plasma were purchased from Wako Pure Chemicals Industries (Japan). All ssDNAs, except for the random ssDNA library with its primer set, were synthesized by Sigma-Aldrich (USA). 2-Morpholinoethanesulfonic acid (MES; Wako, Japan), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimido hydrochloride (EDC; Wako, Japan), Dulbecco’s PBS(−) (Wako, Japan), 10% Tween 20 solution (Bio-Rad, USA), tris(hydroxymethyl)aminomethane (Tris; Wako, Japan), 1 M sodium chloride solution (Wako, Japan), 1 M magnesium chloride solution (Wako, Japan), 1 M sodium hydroxide solution (Agilent Technologies, USA), 0.5 M borate buffer at pH 8.5 ± 0.2 (Polysciences, USA), EDTA disodium salt dihydrate (Wako, Japan), and boric acid (Wako, Japan) were used as received. All solutions were prepared using ultrapure water from a Milli-Q water purification system (Merck Millipore, USA). For the preparation of gels, a 37.5:1 (40%, w/v) acrylamide/bis solution, 2.6% C (Serva Electrophoresis, Germany), ammonium persulphate (Bio-Rad, USA), and N,N,N’,N’-tetramethylethylene (TEMED; Bio-Rad, USA) were used. The loading buffer and a 25-bp DNA stepladder were purchased from Wako (Japan).
Tris hydroxymethyl aminomethane tris
Manufactured by Fujifilm
Sourced in Japan
Tris(hydroxymethyl)aminomethane (Tris) is a chemical compound used as a buffer in various laboratory applications. It maintains a specific pH range in solutions, allowing for the stabilization and optimization of various biochemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Apo transferrin, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Dulbecco s pbs, by Fujifilm (1 mentions)
Bovine serum albumin (bsa), by Fujifilm (1 mentions)
Thrombin from human plasma, by Merck Group (1 mentions)
Ammonium persulphate, by Bio-Rad (1 mentions)
Sodium hydroxide solution, by Agilent Technologies (1 mentions)
Boric acid, by Fujifilm (1 mentions)
Acrylamide bis solution, by Serva Electrophoresis (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
N n dimethylformamide (dmf), by Fujifilm (1 mentions)
N hydroxysuccinimide, by Fujifilm (1 mentions)
Dioxane, by Fujifilm (1 mentions)
2 4 6 trinitrobenzene sulfonic acid, by Tokyo Chemical Industry (1 mentions)
Sulfuric acid, by Fujifilm (1 mentions)
Ethyl acetate, by Fujifilm (1 mentions)
Sodium azide, by Fujifilm (1 mentions)
Sodium dihydrogenphosphate dehydrate, by Fujifilm (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Fujifilm (1 mentions)
4 protocols using tris hydroxymethyl aminomethane tris
1
Protein-DNA Conjugation Protocol
2
Erlotinib Quantification Assay Development
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Erlotinib hydrochloride and O-desmethyl erlotinib were obtained from AdooQ BioScience LLC (Irvine, CA, USA). Ethyl 3,4-bis(2-methoxyethoxy)benzoate (EBMB) was obtained from AK Scientific, Inc (Union City, CA, USA). 2,4,6-Trinitrobenzene sulfonic acid was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Horseradish peroxidase (HRP) and 3,3,5,5-tetramethylbenzidine (TMB) were obtained from Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). Sodium hydroxide, hydrochloric acid, sulfuric acid, sodium dihydrogenphosphate dehydrate, disodium hydrogenphosphate 12-water, sodium azide, tris(hydroxymethyl)aminomethane (Tris), 1-ethyl-3,3-dimethylaminopropyl carbodiimide hydrochloride (EDC), N-hydroxysuccinimide, dioxane, ethyl acetate, N,N-dimethylformamide and bovine serum albumin (BSA) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Hydrogen peroxide (30% in water) was obtained from Nacalai Tesque Inc. (Kyoto, Japan).
3
Synthesis and Characterization of Fluorescent Probes
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1,5-Diamino-3-monooxapentane (DMP; Cat# O0278), 1,8-diamino-3,6-dioxaoctane, (DDO; Cat# B1431) 1,11-diamino-3,6,9-trioxaundecane (DTU; Cat# D3664), N-succinimidyl 3-maleimidopropionate (SMP; Cat# S0427), and N,N′-diisopropylethylamine (DIPEA; Cat# D1599) were purchased from Tokyo Chemical Industry (Tokyo, Japan). 6-Carboxyrhodamine 6G succinimidyl ester (R6G-OSu; Cat# 342) was obtained from AAT Bioquest (Sunnyvale, CA, USA). Tris(hydroxymethyl)aminomethane) (Tris) and adenosine 5′-triphosphate (ATP) were from Wako Pure Chemical Industries (Osaka, Japan). 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was from Dojindo (Kumamoto, Japan). All other chemicals were of reagent grade or higher and purchased from Wako Pure Chemical Industries, Nacalai Tesque (Kyoto, Japan) or Sigma-Aldrich (St. Louis, MO, USA).
4
Purification and Characterization of Hemoglobin Derivatives
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Human Hb used for this work was purified from RBCs provided by the Japanese Red Cross. As reported elsewhere in the literature, 29 a highly purified carbonyl Hb (native Hb) solution was obtained through pasteurization and nanofiltration. DBBF crosslinked Hb (XLHb) was also prepared as reported in the literature. 26, 30 The Hb derivative concentrations were determined using Hemoglobin B-test Wako (Fujifilm Wako Pure Chemical Corp.). Sodium chloride, potassium chloride, disodium hydrogen phosphate (Na 2HPO4), potassium dihydrogen phosphate (KH2PO4), polyethylene glycol 20,000 (20 kDa PEG), ammonium sulfate, Tris(hydroxymethyl) aminomethane (Tris), sucrose, sodium dodecyl sulfate (SDS), 2-mercaptoethanol, bromophenol blue, and Coomassie brilliant blue (Quick CBB) were purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan). Saline (Otsuka normal saline) was purchased from Otsuka Pharmaceutical Co. Ltd. (Tokyo, Japan). Albumin (25% human serum albumin) was purchased from Japan Blood Products Organization (Tokyo, Japan).
Bi-functional maleimide PEG, mal2-PEG (Figure 1; SUNBRIGHT DE-100MA, Lot M153536, Mn 10456, Mw/Mn = 1.02, substitution 90.6%) was purchased from NOF Corp. (Tokyo, Japan).
Bis-(3,5-dibromosalicyl) fumarate (Figure 1; DBBF) was purchased from Abcam plc.
(Cambridge, U.K.).
Bi-functional maleimide PEG, mal2-PEG (Figure 1; SUNBRIGHT DE-100MA, Lot M153536, Mn 10456, Mw/Mn = 1.02, substitution 90.6%) was purchased from NOF Corp. (Tokyo, Japan).
Bis-(3,5-dibromosalicyl) fumarate (Figure 1; DBBF) was purchased from Abcam plc.
(Cambridge, U.K.).
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