Btla pe

Manufactured by BioLegend

Sourced in Germany

BTLA-PE is a lab equipment product from BioLegend. It is a fluorochrome-conjugated antibody that binds to the B and T lymphocyte attenuator (BTLA) protein. The core function of this product is to facilitate the detection and analysis of BTLA expression on cells.

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Lab products found in correlation

2 protocols using btla pe

Comprehensive Immune Phenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies:
(1) BD Biosciences: CD3-H7APC (SK7), CD45RA-Cy7PE (L48), CTLA-4-APC (BNI3),
IFN-g-FITC (B27), CCR7-Alexa700 (150503), CCR5-FITC (2D7/CCR5), CCR4-PE (1G1) and
IL-2-PE (MQ1-17H12), Ki67-FITC (B56); (2) Beckman Coulter: CD27-Alexa680
(IA4CD27), CD127-Cy5PE (R34.34), CD160-PE (BY55), BTLA-PE (J168-540); (3)
BioLegend: PD-1-BV421 (EH12.2H7), 2B4-FITC (CD244, C1.7), IL-17a-Cy5.5PerCP
(BL168), CCR6-Alexa647 (or PE, TG7/CCR6), CD27 Alexa647 (O323), CCR7 BV605
(G043H7), CXCR5 BV421 (J252D4), and CD154-Cy5PE (24–31); (4) Invitrogen:
CD4-Cy5.5PE (S3.5), CD27-QD605 (CLB-27/1), CD8-QD800 (3B5). A biotinylated
anti-PD-1 antibody was obtained from R&D (BAF 1086) and streptavidin-Qdot
655 was obtained from Molecular Probes. Quantum dots and Aqua amine viability dye
were obtained from Invitrogen. CD27-Alexa 594, TNF-a Alexa 594, HLA-DR BV650, and
CD38-Alexa 680 were conjugated in-house.

Paris R.M., Petrovas C., Ferrando-Martinez S., Moysi E., Boswell K.L., Archer E., Yamamoto T., Ambrozak D., Casazza J.P., Haubrich R., Connors M., Ake J., Kim J.H, & Koup R.A. (2015). Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE, 10(12), e0144767.

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Comprehensive Immune Cell Profiling

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GICs and PBMCs were stained for CD3-PE-Cy5.5 (eBioscience), CD4-BV711, CD8-PerCP, CD25-BV605, CD45RA-BV570, CCR7-BV650, CD39-BV421, PD-1-APC-Cy, BTLA-PE, Tim-3-PE-Cy7 (all Biolegend), LAG3 (Enzo Life Sciences, Lörrach, Germany), CTLA4-PE-CF594 (BD) and Live/dead-Aqua dye (Life technologies, Carlsbad, CA) or with isotype controls. Cells were fixed and permeabilized, followed by ICS using Foxp3-FITC (eBioscience) and Ki67-Alexa Fluor 700 (BD) and measured on an LSR Fortessa (BD).

Rammensee H.G., Wiesmüller K.H., Chandran P.A., Zelba H., Rusch E., Gouttefangeas C., Kowalewski D.J., Di Marco M., Haen S.P., Walz J.S., Gloria Y.C., Bödder J., Schertel J.M., Tunger A., Müller L., Kießler M., Wehner R., Schmitz M., Jakobi M., Schneiderhan-Marra N., Klein R., Laske K., Artzner K., Backert L., Schuster H., Schwenck J., Weber A.N., Pichler B.J., Kneilling M., la Fougère C., Forchhammer S., Metzler G., Bauer J., Weide B., Schippert W., Stevanović S, & Löffler M.W. (2019). A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer. Journal for Immunotherapy of Cancer, 7, 307.

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