The extract was purified using reverse phase HPLC instrument (Agilent technologies, CA, USA) equipped with Zorbax C18 column and photodiode array detector. The mobile phase was consisted of solvent A (MilliQ water) and solvent B (HPLC grade acetonitrile with 0.1% trifluoroacetic acid). The lipopeptides were eluted by linearly increasing the percentage of solvent B from 5–95% for 60 min at a flow rate of 0.4 ml/min. About 50 μl of methanol extracted sample and standard Iturin (Sigma, USA) was injected into the column. The HPLC fractions were collected automatically and subjected to mass spectrometry. Electrospray ionization mass spectrometry (ESI-MS) analysis was performed on EVOQ triple quadrupole mass spectrometer (Bruker). ESI-MS conditions were as follows: capillary voltage of 35 V, a spray voltage of 4.5 kV and a capillary temperature of 300 °C. Samples were injected with a syringe at a flow rate of 0.2 ml/min.
Evoq triple quadrupole mass spectrometer
Manufactured by Bruker
Sourced in United States
The EVOQ triple quadrupole mass spectrometer is a high-performance analytical instrument designed for quantitative and qualitative analysis of a wide range of compounds. It utilizes triple quadrupole mass spectrometry technology to provide accurate and sensitive detection and measurement of target analytes in complex matrices.
Automatically generated - may contain errors
Lab products found in correlation
Msws 8, by Bruker (2 mentions)
Iturin, by Merck Group (1 mentions)
Zorbax c18 column, by Agilent Technologies (1 mentions)
Uplc system, by Bruker (1 mentions)
Gemini c18, by Phenomenex (1 mentions)
Supra 40vp field emission scanning electron microscope, by Zeiss (1 mentions)
Dxrxi raman imaging microscope, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 uhplc, by Thermo Fisher Scientific (1 mentions)
6 protocols using evoq triple quadrupole mass spectrometer
1
Purification and Mass Spectrometry of Lipopeptides
2
Quantitative UPLC-MS/MS Analysis of Resveratrol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The UPLC system (Bruker Scientific LLC; Billerica, MA, USA) was equipped with a degasser, an integrated column oven, a binary pump, and an autosampler (CTC-PAL).
Chromatographic separation was achieved through a Kinetex 1.7 µm C18 (50 x 2.1 mm, 100 A) column (Phenomenex Inc; Torrance, CA, USA), connected to a Phenomenex C18 Security Guard ULTRA (2.1 mm) pre-column, which were maintained at 40°C. A gradient mobile phase consisting of a mixture of 5 mM ammonium acetate in water (A) and 0.05% formic acid in acetonitrile: methanol (B) (95:5) was delivered to the column at a flow rate of 0.4 mL/min. The gradient conditions were as follows: 0-0.5 min, 2% B; 0.5-1.7 min, linear gradient 2-98% B; 1.7-3.4 min, 98% B; 3.6 min, 2% B; 3.6-4 min, 2% B.
Quantitation was achieved in the positive ion mode by a Bruker EVOQ triple quadrupole mass spectrometer, equipped with ion spray interface, using a temperature of 400°C and ion spray voltage of 3000 V. The curtain gas, heated probe gas, and nebulizer gas flow were set at 30, 45 and 55 psi, respectively. Detection of the ions was performed in the multiple reaction monitoring (MRM) mode, with the m/z transitions of 213.9 to 185.9 for RES and 220.0 to 192.0 for IS. Quadrupole Q1 and Q3 were set to unit resolutions. The dwell time was 100 msec. The instrument was controlled by the Bruker MSWS-8 software.
Chromatographic separation was achieved through a Kinetex 1.7 µm C18 (50 x 2.1 mm, 100 A) column (Phenomenex Inc; Torrance, CA, USA), connected to a Phenomenex C18 Security Guard ULTRA (2.1 mm) pre-column, which were maintained at 40°C. A gradient mobile phase consisting of a mixture of 5 mM ammonium acetate in water (A) and 0.05% formic acid in acetonitrile: methanol (B) (95:5) was delivered to the column at a flow rate of 0.4 mL/min. The gradient conditions were as follows: 0-0.5 min, 2% B; 0.5-1.7 min, linear gradient 2-98% B; 1.7-3.4 min, 98% B; 3.6 min, 2% B; 3.6-4 min, 2% B.
Quantitation was achieved in the positive ion mode by a Bruker EVOQ triple quadrupole mass spectrometer, equipped with ion spray interface, using a temperature of 400°C and ion spray voltage of 3000 V. The curtain gas, heated probe gas, and nebulizer gas flow were set at 30, 45 and 55 psi, respectively. Detection of the ions was performed in the multiple reaction monitoring (MRM) mode, with the m/z transitions of 213.9 to 185.9 for RES and 220.0 to 192.0 for IS. Quadrupole Q1 and Q3 were set to unit resolutions. The dwell time was 100 msec. The instrument was controlled by the Bruker MSWS-8 software.
3
SERS Characterization of Ag-Capped Silicon Nanopillars
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All SERS measurements were performed with a DXRxi Raman Imaging Microscope (Thermo Fisher Scientific Inc., Waltham, MA, USA). The optical microscope was coupled to a spectrometer 5 cm -1 FWHM and ±2 cm -1 wavenumber accuracy. SERS spectra were collected at 780 nm with a laser power of 10 mW, with a 10x objective lens, 50 µm slit and an estimated laser spot of 3.6 µm diameter.
All the spectra were collected three times for 0.05 s in each spot. SERS maps were collected on the whole surface of each chip (three maps/three times scanning on each chip), with a 100 µm collection step and an overall collection time of 3 min. Also, triplicate measurements were acquired for all experiments.
Liquid chromatography was carried out on an Ultimate 3000 uHPLC (Thermo Fisher, Waltham, MA, USA) instrument. The separation was performed on a Gemini, C18 (3µm, 2 mm × 100mm) col-umn from Phenomenex (Torrance, CA, USA). ACN/water (70:30 v/v) was used as a mobile phase.
Mass spectra were acquired by a Bruker EVOQ triple quadrupole mass spectrometer (Bruker Daltonics, Bremen, Germany) equipped with an ESI interface in the positive-ion mode. A scanning electron microscope (SEM) (Zeiss Supra 40VP field emission scanning electron microscope) was used for the characterization of the Ag-capped silicon nanopillars.
All the spectra were collected three times for 0.05 s in each spot. SERS maps were collected on the whole surface of each chip (three maps/three times scanning on each chip), with a 100 µm collection step and an overall collection time of 3 min. Also, triplicate measurements were acquired for all experiments.
Liquid chromatography was carried out on an Ultimate 3000 uHPLC (Thermo Fisher, Waltham, MA, USA) instrument. The separation was performed on a Gemini, C18 (3µm, 2 mm × 100mm) col-umn from Phenomenex (Torrance, CA, USA). ACN/water (70:30 v/v) was used as a mobile phase.
Mass spectra were acquired by a Bruker EVOQ triple quadrupole mass spectrometer (Bruker Daltonics, Bremen, Germany) equipped with an ESI interface in the positive-ion mode. A scanning electron microscope (SEM) (Zeiss Supra 40VP field emission scanning electron microscope) was used for the characterization of the Ag-capped silicon nanopillars.
4
Quantitative Plasma Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were extracted according to the method developed and described by Legacki and coworkers (2016) . Briefly, the P4-d9 internal standard was added to all plasma samples and the samples were extracted with methyl tert-butyl ether (1:5). Calibrators and quality control samples were prepared in charcoal-stripped plasma. Calibrators ranged from 0.1 to 100 ng/mL and four levels of quality control (QC) samples (0.6, 1.5. 20 and 80 ng/mL) were prepared along with the samples. The plasma samples were shaken for 15 min and centrifuged at 3000 g for 5 min. The resulting supernatant was transferred into a 12 × 75 glass tube and dried using a Zymark TurboVap concentrator (Hopkinton, MA, USA) at 45°C with N2. Samples were reconstituted with 200 µL of 50:50 water and methanol. Quantitation of analytes was determined by linear regression analysis of the ratio of analyte area to the area of designated internal standard. Tandem mass spectral detection was developed using a Bruker EVOQ Triple Quadrupole Mass Spectrometer (Billerica, MA, USA). Calculations were made using the Bruker software. A minimum of six-point calibration curve and a maximum of ten points were used depending on the concentration of each analyte.
5
Quantitative Mass Spectrometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extracted samples were reconstituted with 200 µL of 50:50 water and methanol. Calibration standards were run at the beginning and at the end of each sample set; QC samples were run at the beginning of each sample set. Quantitation of analytes was determined by linear regression analysis of the ratio of the area of analyte to internal standards, which were chosen for each analyte based on structure and chromatographic retention time. Tandem mass spectral detection was accomplished using a Bruker EVOQ triple quadrupole Mass Spectrometer (Billerica, MA, USA). Calculations were done using the Bruker software (Billerica, MA, USA). A minimum of a six-point calibration curve and maximum of ten points were used depending on the concentration range of each analyte.
6
UPLC-MS/MS Protocol for Metabolite Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The UPLC-MS/MS system consisted of a Bruker EVOQ triple quadrupole mass spectrometer, attached to the Bruker Advance UPLC system with an integrated column oven, degasser, and a CTC PAL autosampler. The system was controlled and the data acquired and quantified by the Bruker MSWS 8 software. The chromatographic separation was achieved using a Phenomenex Kinetex 1.7 µm C18 (100 A, 50 x 2.1 mm) column, connected to a Phenomenex C18 SecurityGuard ULTRA (2.1 mm) pre-column and maintained at 40 0 C. Samples (2 µL) were injected onto the column and eluted at a flow rate of 0.2 mL/min under gradient conditions consisting of solvent A (5 mM ammonium formate: formic acid, 100:0.05) and solvent B (acetonitrile: methanol: formic acid, 95:5:0.05). Gradient conditions were as follows: 0-0.5 minutes, 10% B; 0.5-4 min, linear gradient 10-90% B; 4-7 min, 90% B; 7 min, 10% B; 7-9 min,
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!