Ampicillin, 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT), trypan blue, cimetidine, and hydrotalcite were purchased from Sigma (Sigma-Aldrich Inc., MO, USA). Cell culture medium and reagents, such as RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin, and trypsin-EDTA were purchased from GIBCO (Invitrogen Inc., NY, USA). AnaeroPack Campylo, PGE2 assay kit was obtained from Mitsubishi Gas Cjemical Co., Inc. and R&D Systems, Inc. respectively. HCl, EtOH, and other solvents were purchased from Duksan pure Chemical Co. Ltd. (Kyunggi-do, Korea). All other reagents were the pharmaceutical or analytical grade. UV-spectrophotometric plate reader (ASYS UVM340), centrifuge 5810R (eppendorf), pH meter (IQ scientific instruments Inc), CO2 incubator (Forma scientific), inverted microscope (Olympus), liquid nitrogen dewars (CHART MVE) were used as laboratory equipments.
Hydrotalcite
Manufactured by Merck Group
Sourced in United States
Hydrotalcite is a synthetic clay-like material composed of magnesium, aluminum, and hydroxide ions. It has a layered structure and is commonly used as a laboratory adsorbent, catalyst, and catalyst support in various chemical and pharmaceutical applications.
Automatically generated - may contain errors
Lab products found in correlation
Cimetidine, by Merck Group (2 mentions)
Ampicillin, by Merck Group (2 mentions)
Trypan blue, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Inverted microscope, by Olympus (1 mentions)
Centrifuge 5810r, by Eppendorf (1 mentions)
Phenol red, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Sodium nitrate, by Merck Group (1 mentions)
Sepiolite, by Merck Group (1 mentions)
Pfdtes, by Gelest (1 mentions)
Halloysite, by Merck Group (1 mentions)
5 protocols using hydrotalcite
1
Cytotoxicity Evaluation of Ampicillin
2
Synthesis of Silicon Oxycarbide Ceramic
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To synthesize silicon oxycarbide, three polymeric precursors were chosen: a linear polymethylhydrosiloxane [PHMS, (CH3)3SiO{(CH3)HSiO}nSi(CH3)3, viscosity: 15–40 mPa.s (20 °C), CAS Number 63148-57-2, Sigma Aldrich, USA] for getting the basic structure of ceramic, vinyl terminated polydimethylsiloxane [PDMS,CH2CH{Si(CH)2O}nSi(CH3)2 CHCH2, viscosity:850–1150 mPa.s (25 °C), CAS Number 68083-19-2, Sigma Aldrich, USA] as a sacrificial pore former, cyclic 2,4,6,8-tetramethyl-2,4,6,8 tetravinlycyclotetrasiloxane [TMTVS, C12H28O4Si4, CAS Number 2554-06-05, Sigma Aldrich, USA) with SiC=C moieties] for increasing the crosslinking density. Hydrotalcite [LDH with formula {Mg6Al2(CO3) (OH)16·4H2O}, CAS Number 11097-59-9, Sigma-Aldrich, St. Louis, MO, USA], was used in the as-received condition for aligning the pores. Platinum (0)-1,3-divinyl-1,1,3,3-tetramethyldisiloxane complex solution in xylene, Pt2%, [O{Si(CH3)2CH=CH2}2 Pt, Sigma Aldrich, USA] was used as the catalyst for the crosslinking reactions. Graphene (CAS NO.: 7782-42-5, United Nanotech Innovations Pvt. Ltd., Bangalore, India) with the specific surface area (SSA) of 300 m2/g was taken for dispersing in the polymeric precursor mixture. All materials are used without further purification.
3
Indomethacin Anti-Inflammatory Assay
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Indomethacin, ATP, phenol red, carboxymethyl cellulose (CMC), and the positive controls, ascorbic acid, hydrotalcite, ampicillin, and cimetidine were purchased from Sigma (Sigma-Aldrich Inc., MO, USA). HCl, EtOH, and NaOH were purchased from the Duksan Pure Chemical Co. Ltd. (Kyunggi-do, Korea). All other reagents and solvents used were of pharmaceutical or analytical grade.
4
Synthesis and Characterization of Oxazine-based Compounds
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Oxazine 170 perchlorate (O17), ethyl cellulose (EC), Devada’s alloy (DA)-100 mesh, hydrotalcite (HT), sodium nitrate, and sodium nitrite were purchased from Sigma Aldrich Chemical Company (Sigma Aldrich Chemical Co., Seoul, Korea). Other analytical-grade chemicals, such as sodium phosphate, potassium phosphate, sodium chloride, potassium chloride, sodium hydroxide, hydrochloric acid, sodium bicarbonate, magnesium sulfate, sodium sulfate, and calcium chloride, were used without further purification.
5
Preparation and Characterization of Modified Clays
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PAL was purchased from Jiangsu, China. Kaolinite and vermiculite were purchased from Fujian, China. Illite and rectorite were purchased from Hubei, China. Mica was purchased from Hebei, China. Na+-MMT was purchased from Shandong, China. Ca2+-MMT and Li+-MMT was purchased from Henan, China. Halloysite, diatomite, hydrotalcite and sepiolite were purchased from Sigma-Aldrich. Laponite RD was supplied by Southern Clay Products, Inc. For acid activation, the clays (15 g) were suspended in the 2 M HCl aqueous solutions (150 mL) at room temperature and magnetically stirred for 2 h. The acid activated clays were washed with deionized water until pH 6, and then dried at 105 °C to a constant weight. The clays were sifted using a 200 mesh sieve. Glass slides (24 × 50 mm) were purchased from Menzel, Germany. PFDTES (97%) and TEOS (99.9%) were bought from Gelest. Ethanol, ammonia, CH2I2, toluene n-dodecane, n-hexadecane and n-decane were bought from China National Medicines Co. Ltd.
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