Alizarin red s

Manufactured by Olympus

Sourced in Japan

Alizarin red S is a chemical compound used as a dye and staining agent in various laboratory applications. It is commonly used for the detection and identification of calcium deposits in tissues and cells. Alizarin red S produces a bright red-orange color when it binds to calcium ions, making it a useful tool for visualizing and analyzing calcium-containing structures.

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Lab products found in correlation

Alizarin red s, by Merck Group (2 mentions) Szx zb7, by Olympus (1 mentions) Alcian blue 8gx, by Merck Group (1 mentions) Alizarin red s ar, by Merck Group (1 mentions) Inverted microscopy, by Olympus (1 mentions) Endothelial tube formation assay, by Cell Biolabs (1 mentions) Bx60 microscope, by Olympus (1 mentions) 16 well chamber slide, by Thermo Fisher Scientific (1 mentions) Osteogenic differentiation medium, by ScienCell (1 mentions) Alizarin red s staining, by Merck Group (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Paraformaldehyde (pfa), by Fujifilm (1 mentions) Ix41 microscope, by Olympus (1 mentions) Nex 5 t, by Sony (1 mentions) Alizarin red s, by Nacalai Tesque (1 mentions) Infinite 200 pro plate reader, by Tecan (1 mentions)

Close spelling variants of this product

Alizarin red (6 citations)

4 protocols using alizarin red s

Embryonic Skeletal Mineralization Quantification

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Embryos were euthanized using a 300 mg/L tricaine methanesulfonate (Merk Life Science S.r.l., Milano, Italy) solution [33 (link)] and fixed in 3.5% formaldehyde/0.1 M sodium phosphate buffer. Soon after, double acid-free staining with Alcian Blue 8GX (Sigma) and alizarin red S (ARS, Sigma) [34 (link)] was applied to stain cartilage and bone tissue, respectively. The quantification of osteogenesis level was measured as vertebral mineralization rate (N.V./L.), calculated as the number of mineralized vertebral bodies (N.V., positive for alizarin red S staining) normalized for the length of each embryo (L.).
All embryos were examined under a light/fluorescence stereomicroscope (SZX-ZB7 Olympus), acquiring images with a Discovery CH30 camera (Tiesselab), which were analyzed with ISC Capture software to take measurements.

Carnovali M., Ciavatta M.L., Mollo E., Roussis V., Banfi G., Carbone M, & Mariotti M. (2022). Aerophobin-1 from the Marine Sponge Aplysina aerophoba Modulates Osteogenesis in Zebrafish Larvae. Marine Drugs, 20(2), 135.

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Alizarin Red S Staining of Mineralized Osteogenic MSCs

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MSCs were cultured with osteogenic induction medium in the presence or absence of indoxyl sulfate and p-cresol as previously described. The production of bone matrix containing calcium crystals was assessed by alizarin red S staining at day 14 of differentiation. Cells were washed twice with PBS, fixed by incubating with 10% (v/v) formaldehyde at room temperature for 15 minutes, and washed twice with distill water. At this stage, the cells were incubated with 1 ml 40 mM alizarin red S (pH 4.1, Sigma-Aldrich, USA) at room temperature for 20 minutes with shaking. The excess dye was removed by washing with distill water. The alizarin red S staining was assessed and photographed under inverted microscopy (Olympus, Japan).

Kamprom W., Tawonsawatruk T., Mas-Oodi S., Anansilp K., Rattanasompattikul M, & Supokawej A. (2021). P-cresol and Indoxyl Sulfate Impair Osteogenic Differentiation by Triggering Mesenchymal Stem Cell Senescence. International Journal of Medical Sciences, 18(3), 744-755.

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Multipotential Differentiation of DPSCs

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Characterization of DPSCs (3rd–5th passage) was examined by multipotential differentiation. Angiogenic differentiation was evaluated using an endothelial tube formation assay (Cell Biolabs, San Diego, CA, USA) according to the manufacturer’s instructions. In brief, 1.5 × 10⁴ cells cultured in angiogenic induction medium for 10 days were seeded in a 16-well chamber slide (Thermo Fisher Scientific) coated with 50 µL extracellular matrix (ECM) gel. After 4 h, the cells were incubated with 1× Staining solution and imaged by an Olympus FV1000 confocal microscope (Olympus, Center Valley, PA, USA). For odontogenesis, 1.5 × 10⁴ cells were cultured with osteogenic differentiation medium (ScienCell, Carlsbad, CA, USA) in a 6-well plate, and Alizarin Red S staining (Sigma-Aldrich) was used to evaluate the cellular calcium deposit at 10 and 20 days. The cells fixed in 4% formaldehyde were stained with 40 mM Alizarin Red S for 30 min (min) and imaged by an Olympus BX60 microscope (Olympus).

Ganesh V., Seol D., Gomez-Contreras P.C., Keen H.L., Shin K, & Martin J.A. (2022). Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. International Journal of Molecular Sciences, 24(1), 466.

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Alizarin Red-S Staining of SHED Cells

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SHED were fixed with 4% paraformaldehyde (Wako Pure Chemical Industries) in 0.1 M sodium phosphate buffer (pH 7.4) for 10 min at room temperature, and washed three times with phosphate-buffered saline. The cells were then rinsed with dH 2 O and stained with 1% Alizarin Red-S (pH 4.2; Sigma-Aldrich) for 30 s. Cells were imaged using an ES-2200 scanner (EPSON, Nagano, Japan). To quantify Alizarin Red-S staining, 10% (v/w) cetylpyridinium chloride (Nakarai tesque) in 10 mM sodium phosphate buffer (pH 7.0) was used to extract Alizarin Red-S, the absorbance of which was measured at 570 nm using an Infinite 200 PRO plate reader (Tecan, M€ annedorf, Switzerland). To measure the Alizarin Red-S stained area, three images were randomly taken from three experiments using an IX41 microscope (Olympus, Tokyo, Japan) equipped with a digital camera NEX-5T (Sony, Tokyo, Japan) with an NY-1S adaptor (MeCan Imaging, Saitama, Japan), and were analyzed using MetaMorph software version 7.8.2.0 (Molecular Devices, CA, USA).

Kato H., Han X., Yamaza H., Masuda K., Hirofuji Y., Sato H., Pham T.T.M., Taguchi T, & Nonaka K. (2017). Direct effects of mitochondrial dysfunction on poor bone health in Leigh syndrome. Biochemical and biophysical research communications, 493(1).

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