Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAwayTM one-step mycoplasma detection kit (Yeasen). Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLETM express (GIBCO). 10 μM Peramivir (M3222, AbMole BioScience), 1 μM Nucleozin (A3670, Apexbio), 50 μM PYC-12, 20 μM RO3306, 10 μM WH-L50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
Mycaway one step mycoplasma detection kit
Manufactured by Yeasen
MycAwayTM is a one-step mycoplasma detection kit. It is designed to detect the presence of mycoplasma contamination in cell culture samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Neuroeasy maintenance medium, by Cellapy (2 mentions)
Nucleozin, by Apexbio (2 mentions)
Peramivir, by AbMole (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Essential 8 medium, by Thermo Fisher Scientific (2 mentions)
Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (2 mentions)
2 protocols using mycaway one step mycoplasma detection kit
1
Cell Culture and Treatment Conditions
2
Cell Culture Protocols for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAway TM one-step mycoplasma detection kit (Yeasen).
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!