Pooled primary human periodontal ligament fibroblasts (hPLFs) were obtained from a commercial source (ScienCell Research Laboratories) and were cultured as previously described [33 (link), 34 (link)]. Briefly, the hPLFs were cultured in fibroblast medium [DMEM (high glucose) supplemented with 10% fetal bovine serum, 1 mM L-glutamine, and 1% penicillin/streptomycin]. Passages 4–6 hPLFs were used in all subsequent experiments.
Hplfs
Manufactured by ScienCell
HPLFs are primary human lung fibroblast cells. They are isolated from normal human lung tissue and are cryopreserved at the second passage to maintain their proliferative capacity and original phenotype.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hplfs
1
Culturing Human Periodontal Ligament Fibroblasts
2
Osteogenic Differentiation of Periodontal Ligament Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human periodontal ligament fibroblasts (HPLFs) (ScienCell, Cat. No. 2630) were cultured in fibroblast medium (ScienCell, Cat. No. 2301), which consisted of 100 mL of basal medium, 10 mL of fetal bovine serum (FBS, Cat. No. 0010), 5 mL of fibroblast growth supplement (FGS, Cat. No. 2352), and 5 mL of antibiotic solution (P/S, Cat. No. 0503). After embedding the cells within the collagen scaffold, we cultured them further in osteogenic differentiation medium (ScienCell, Cat. No. 7531) [30 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!